Biological Molecule Analysis
Biological Molecule Analysis - Related Videos
11:46
Single Molecule Analysis of Laser Localized Psoralen Adducts
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2017
Lasers are frequently used in studies of the cellular response to DNA damage. However, they generate lesions whose spacing, frequency, and collisions with replication forks are rarely characterized. Here, we describe an approach that enables the determination of these parameters with laser localized interstrand crosslinks.
07:28
Synthetic Biology
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2023
This video presents synthetic biology and its role in bioengineering. Synthetic biology refers to the methods used to genetically modify organisms in order to make them capable of producing large quantities of a product. This product could be a protein that the cell already makes or a new protein that has been encoded in a newly-inserted DNA sequence. Here, we discuss how an organism's genetic material is modified using transformation or transfection. Then, the process is shown in the...
07:00
Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
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Cited by 25 •
2013
This protocol describes an experimental procedure for performing Fluorescence in situ Hybridization (FISH) for counting mRNAs in single cells at single-molecule resolution.
09:27
An Introduction to Molecular Developmental Biology
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2023
Molecular signals play a major role in the complex processes occurring during embryonic development. These signals regulate activities such as cell differentiation and migration, which contribute to the formation of specific cell types and structures. The use of molecular approaches allows researchers to investigate these physical and chemical mechanisms in detail. This video will review a brief history of the study of molecular events during development. Next, key questions asked by molecular...
01:57
What is Conservation Biology?
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2019
Conservation biology is a scientific field that focuses on the preservation of biodiversity in order to protect ecosystems while meeting the needs of the human population. Humans require properly functioning ecosystems to maintain our supply of natural resources, including food, medicines, and building materials. Ecosystems also perform critical services, such as purifying our air and water. A large body of evidence indicates that such ecosystem services depend on biodiversity. Furthermore,...
FAQs
Analytical methods distinguish biomolecules by measurable properties such as size, charge, chemical composition, or binding affinity. These differences allow researchers to separate related molecules or detect selective interactions within a sample. The resulting patterns help determine which molecules are present and support measurements of their abundance, structure, or functional relationships.
Each measurement addresses a different biological question. Identifying a molecule establishes what is present, while measuring abundance shows how much occurs. Structural information describes molecular organization, and functional analysis connects a molecule to activity or interaction. Considering these dimensions together gives a more informative view of molecular changes than relying on a single measurement.
Molecular analysis can connect changes in one class of biomolecule with effects in another. For example, measurements of nucleic acids, proteins, lipids, or carbohydrates can be interpreted alongside changes in gene expression, protein interactions, or metabolic activity. These relationships help researchers examine cellular pathways rather than treating each molecule as an isolated observation.
A typical workflow begins with sample preparation, which makes the biological material suitable for examination. Researchers then separate or otherwise organize molecules according to relevant properties, detect the molecules or signals of interest, and characterize the resulting data. This sequence supports reliable interpretation of molecular identity, abundance, structure, and function.
The biological question determines which molecular feature requires emphasis. A study of gene expression prioritizes nucleic-acid measurements, whereas investigation of protein interactions focuses on molecular binding relationships. Questions about metabolic changes may require attention to lipids, carbohydrates, or related molecular patterns. Selecting the relevant target and property keeps the analysis connected to its purpose.
The resulting molecular information supports disease research, drug development, diagnostics, and biotechnology. In basic biology, it helps investigators follow cellular pathways and understand how molecular changes influence cells and organisms. Because the approach can examine multiple biomolecule classes, it also provides a foundation for connecting molecular observations with broader biological processes and outcomes.