Biological Oxygen Measurement
Biological Oxygen Measurement - Related Videos
10:38
Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
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Cited by 2 •
2010
We present principles of oxygen measurements by phosphorescence quenching and review design of porphyrin-based dendritic nanosensors for oxygen imaging in biological systems.
08:58
Cerebral Blood Oxygenation Measurement Based on Oxygen-dependent Quenching of Phosphorescence
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Cited by 27 •
2011
We present an experimental procedure for measuring the partial pressure of oxygen (pO2) in cerebral vasculature based on oxygen-dependent quenching of phosphorescence. Animal preparation and imaging procedures were outlined for both large field of view CCD-based imaging of pO2 in rats and 2-photon excitation based imaging of pO2 in mice.
01:19
Special considerations while measuring oxygen saturation
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2024
Assessing respiratory rate concurrently with pulse measurement is fundamental to patient care, providing valuable insights into the patient's respiratory function. The normal breathing rate for an adult usually falls within a normal range of 12 to 20 breaths per minute. Abnormal respiratory rates can signal underlying health conditions or the need for immediate intervention. Ensuring accuracy in vital sign recordings while prioritizing patient comfort and minimizing anxiety is important.
04:02
Measurement of Oxygen Consumption Rate in Acute Mouse Brain Slices
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2025
Source: Zhi, L. et al., Measurement of Oxygen Consumption Rate in Acute Striatal Slices from Adult Mice. J. Vis. Exp. (2022).This video demonstrates how to measure mitochondrial oxygen consumption rate (OCR) in mouse striatal brain slices. It details the steps for preparing the tissue plate and sensor cartridge, loading them into the flux analyzer, and explains the cellular processes underlying OCR patterns.
08:17
Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
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Cited by 24 •
2011
Fluorescent nanoparticles produced in our lab are used for imaging ion concentrations and ion fluxes in biological systems such as cells during signaling and interstitial fluid during physiological homeostasis.
FAQs
Electrodes and optical sensors translate oxygen availability into different signal types. At an electrode, oxygen reduction generates a measurable electrical signal. Optical systems detect oxygen-dependent changes in fluorescence or luminescence. Calibration then connects either signal to oxygen level, allowing measurements from living systems to be expressed as concentration or used to calculate consumption.
Calibration is essential because the raw sensor response is not itself an oxygen concentration. Relating the electrical, fluorescence, or luminescence signal to oxygen levels makes results interpretable and comparable. This step supports reliable oxygen profiles and consumption estimates, whether the experiment examines cells, tissues, microorganisms, aquatic systems, or a bioreactor.
Oxygen profiles describe how oxygen levels vary within the measured biological setting, while consumption rates indicate how quickly oxygen is being used. Together, they connect an observed signal with respiration, metabolism, and cellular function. Changes under environmental conditions can therefore expose altered physiology rather than merely reporting a single oxygen value.
A concentration measurement reports the oxygen level present at a particular measurement, whereas a consumption measurement focuses on oxygen use by the living system. The first helps characterize oxygen availability; the second provides a readout of respiratory or metabolic demand. Selecting between them depends on whether the study emphasizes conditions, activity, or both.
A basic workflow begins by choosing an oxygen-sensitive electrode or optical sensor for the living system, calibrating its signal against oxygen levels, and then recording the biological sample or environment. Researchers can analyze the resulting measurements as oxygen concentrations, profiles, or consumption rates, depending on the question being investigated.
The method can be applied across microorganisms, mitochondria, tissues, aquatic ecosystems, and bioreactors. In each setting, oxygen data provide a way to examine biological activity in its relevant environment. This broad range makes the approach useful for connecting microbial growth, cellular energy processes, tissue metabolism, ecosystem conditions, and production performance.
Measurements can reveal changes in mitochondrial activity, tissue metabolism, and broader physiological responses. Comparing oxygen profiles or consumption rates under different conditions can show how living systems respond during disease-related investigations or after exposure to a drug. The resulting data provide functional evidence about altered cellular or tissue behavior.
In aquatic ecosystems, oxygen measurements characterize environmental oxygen conditions and biological activity. In bioreactors, they help monitor performance and support optimization of biological production processes. Oxygen profiles and consumption rates are especially useful because they connect system conditions with the activity of the organisms or cells carrying out the process.