Biotinylated Antisense Probes
Biotinylated Antisense Probes - Related Videos
06:15
Biotinylated Cell-Penetrating Peptide Probe to Detect Protein-Protein Interactions
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2025
This video demonstrates a technique that uses biotinylated cell-penetrating peptides to detect intracellular protein-protein interactions. The cell-penetrating peptides facilitate the conjugated peptide to enter a cell and specifically bind to its interacting proteins. The biotin, after binding to avidin, allows purification of the interacting proteins’ complex from a cell lysate.
09:13
Cell-surface Biotinylation Assay
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2023
A cell can regulate the amount of particular proteins on its cell membrane through endocytosis, following which cell surface proteins are effectively sequestered in the cytoplasm. Once within a cell, these surface proteins can be either destroyed or “recycled” back to the membrane. The cell surface biotinylation assay provides researchers with a way to study these phenomena. The technique makes use of a derivative of the small molecule biotin, which can label surface proteins and then be...
11:32
Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
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Cited by 20 •
2017
Here, we detail the method of Sequencing of Psoralen crosslinked, Ligated, and Selected Hybrids (SPLASH), which enables genome-wide mapping of intramolecular and intermolecular RNA-RNA interactions in vivo. SPLASH can be applied to study RNA interactomes of organisms including yeast, bacteria and humans.
11:33
Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
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Cited by 86 •
2009
Microinjection is a well-established and effective method for introducing foreign substances into fertilized zebrafish embryos. Here, we demonstrate a robust microinjection technique for performing mRNA overexpression, and morpholino oligonucleotide gene knockdown studies in zebrafish.
06:43
In Vivo Proximity Biotinylation for Protein Interaction Studies in Paramecium tetraurelia
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Cited by 1 •
2025
The protocol presents a method for in vivo covalent attachment of biotin to proteins based on their proximity to a biotin ligase fused to a protein of interest. This modification allows for a selective enrichment of the proteins using streptavidin beads as needed in protein interaction studies.
FAQs
The antisense sequence directs recognition by matching a complementary RNA or DNA sequence through base pairing. This sequence-dependent interaction gives the probe its target specificity, allowing researchers to distinguish a selected gene or transcript from other nucleic acids. The resulting binding pattern can then be converted into a detectable signal for locating or measuring the target.
Biotin provides a molecular attachment point for streptavidin or avidin, which can be linked to fluorescent, enzymatic, or other reporter molecules. Once the probe has hybridized with its complementary sequence, these binding partners connect the target to a detectable output. The label therefore couples nucleic-acid recognition to visualization or measurement without changing the probe’s antisense sequence.
Useful detection depends on two linked features: complementary base pairing selects the nucleic-acid target, and the biotin-associated reporter produces an observable or measurable signal. Because the target can be RNA or DNA, the same labeling strategy supports different biological questions. The readout may reveal where a sequence occurs or provide evidence for its presence in an assay.
In situ hybridization uses the probe’s complementary sequence to identify a selected RNA or DNA target within a biological sample. The biotin label then supports reporter-based visualization, allowing the target’s location to be examined in its cellular or tissue context. This makes the approach useful for mapping cellular RNA and studying patterns of gene expression.
In Northern blotting, a labeled probe can support detection or measurement of a specific transcript through complementary recognition and reporter-generated signal. In capture assays, the same biotin and streptavidin or avidin interaction can connect the target-associated probe to a detectable system. Together, these applications extend the method from spatial visualization to analytical nucleic-acid measurement.
These probes help researchers investigate where genes or transcripts are found and how their patterns change in biological settings. Applications described for them include examining developmental processes, disease-associated changes, and molecular regulation. By linking sequence recognition with visual or measurable detection, they provide a way to connect nucleic-acid presence with broader cellular and biological processes.