Bone Function
Bone Function - Related Videos
08:49
Models of Bone Metastasis
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Cited by 115 •
2012
Animal models are frequently utilized to study cancer metastasis to bone. In this protocol we will describe two common methods of tumor inoculation for bone metastasis studies and briefly describe some of the analyses utilized to monitor and quantify these models.
07:28
Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments
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Cited by 242 •
2013
We describe a protocol for isolation and purification of neutrophils from mouse bone marrow by density gradient centrifugation and for neutrophil labeling using CellTracker dyes. This represents a simple, fast, reproducible and economical method for obtaining large numbers of neutrophils for downstream functional studies or adoptive transfer and tracking experiments.
07:17
Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation
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Cited by 293 •
2016
Here we present a protocol for the dissection of hind limb long bones (femurs and tibiae) from the laboratory mouse. We further describe a rapid technique for bone marrow isolation from these bones that utilizes centrifugation for removal of bone marrow from the bone marrow space.
14:43
Differentiating Functional Roles of Gene Expression from Immune and Non-immune Cells in Mouse Colitis by Bone Marrow Transplantation
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Cited by 2 •
2012
Bone marrow transplantation provides a way to change the genotype of the bone marrow derived cells. If the gene of interest is expressed in both bone marrow derived cells and non-bone marrow derived cells, bone marrow transplantation can change the bone marrow derived cells to a different genotype without changing the non-bone marrow derived cell genotype.
03:56
Bone Marrow Aspiration: A Method to Obtain Bone Marrow to Examine Cell Morphology
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2023
This video describes the technique of aspirating bone marrow from iliac spine and smear preparation for biopsy. In the example, we perform bone marrow aspiration from an acute myeloid leukemia (AML) patient and examine the cells to check for measurable residual disease (MRD), an important prognostic biomarker.
FAQs
Collagen and mineral crystals contribute complementary properties within bone matrix. Collagen provides flexibility, while mineral crystals add strength. Their combination allows skeletal tissue to support loads without behaving as either a purely rigid or purely flexible material. This relationship helps explain why changes in matrix composition can affect structural performance.
Remodeling couples cellular activity to changing demands on the skeleton. Remodeling cells continuously remove older matrix and replace it, with mechanical stress and hormonal signals influencing the process. This ongoing adjustment helps bones respond to their physical environment and maintain tissue over time rather than remaining unchanged.
Bone marrow within the skeleton supports blood cell production and immune function. This means skeletal tissues contribute to systems beyond movement and support. In biology, that connection makes the skeleton relevant when studying how structural tissues interact with blood-forming and immune processes through its marrow-based activity.
Bone function becomes especially informative in studies of growth, fracture healing, and osteoporosis because these conditions reflect changes in how skeletal tissue develops, repairs itself, or is preserved. Together, remodeling, matrix composition, mechanical stress, and hormonal signals provide a framework for connecting normal roles with skeletal disorders.
Understanding bone function informs biomechanics research by linking skeletal roles to the interaction between matrix properties and mechanical stress. It also supports studies of how physical forces relate to the skeleton's structure and ongoing remodeling. These applications connect basic biology with research designed to explain skeletal performance.
In regenerative medicine, bone function provides a framework for studying how skeletal tissue might be restored or preserved. The same biological context is relevant to fracture healing and to treatments aimed at maintaining skeletal health. Considering collagen, mineral crystals, remodeling cells, and marrow helps connect these research goals to bone as living tissue.