Capillary Tube Pulling
Capillary Tube Pulling - Related Videos
01:18
Rise of Liquid in a Capillary Tube
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2023
When very thin cylindrical tubes, called capillaries, are dipped in a liquid, the liquid rises or falls in the tube compared to the surrounding liquid. This phenomenon is called capillary action. Capillary action occurs due to the combination of two opposing forces: the cohesive forces of the liquid, which cause it to stick to itself and form a rounded shape, and the adhesive forces between the liquid and the walls of the container, which cause the liquid to be attracted to the container walls.
07:38
Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit
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Cited by 24 •
2015
Milk is a primary source of nutrition for the neonate. Analysis of milk components may provide insight into maternal factors that affect offspring health. This protocol describes a manual method of collecting milk samples from the lactating rat, which can then be used for further downstream analysis.
07:51
Pull-down of Calmodulin-binding Proteins
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Cited by 19 •
2012
Calmodulin (CaM) pull-down assay is an effective way to investigate the interaction of CaM with various proteins. This method uses CaM-sepharose beads for efficient and specific analysis of CaM-binding proteins. This provides an important tool to explore CaM signaling in cellular function.
08:50
Capillary Electrophoresis (CE)
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2023
Source: Laboratory of Dr. B. Jill Venton - University of Virginia Capillary electrophoresis (CE) is a separation technique that separates molecules in an electric field according to size and charge. CE is performed in a small glass tube called a capillary that is filled with an electrolyte solution. Analytes are separated due to differences in electrophoretic mobility, which varies with charge, solvent viscosity, and size. Traditional electrophoresis in gels is limited in the amount of voltage...
14:53
Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
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Cited by 2 •
2017
Here, we present a comprehensive capillary zone electrophoresis protocol for the assessment of intrinsic physicochemical heterogeneity of monoclonal antibodies as a quality attribute.
FAQs
Heat softens the glass, allowing the puller to reshape the tube rather than fracture it. Once the material reaches a workable state, controlled tension separates the softened region into two tapered sections. The balance between heating and pulling determines how smoothly the glass narrows, which directly affects whether the finished tool can contact or enter a microscopic biological sample precisely.
The final form depends on four linked variables: heat, pulling force, timing, and the properties of the glass. Heat controls when the tube becomes workable, while force acts on that softened region. Timing coordinates those actions, and glass characteristics influence how it responds. Together, these factors determine the taper and opening needed for a particular biological task.
Programmable control improves reproducibility by applying the selected heating, force, and timing conditions in a controlled sequence. Reproducible tips help standardize how fluids are delivered, how electrodes perform during electrical recordings, and how tools interact with cells. This consistency is especially important when experiments require comparable manipulation of individual cells or subcellular structures.
A basic workflow starts with a glass capillary placed in a programmable puller. The instrument heats the tube until the glass softens, then applies tension to draw the softened section apart. This produces two narrow tips from the original tube. Researchers can then use the resulting capillaries as tools for cell manipulation, injection, recording, or microscopic sampling.
Pulled capillaries can function as microinjection needles, patch-clamp electrodes, cell manipulators, or sampling probes. As needles, they support fluid delivery; as electrodes, they support electrical recordings. Other forms enable direct handling of cells or sampling of microscopic material. The same shaping process therefore supports distinct workflows involving manipulation, measurement, and sampling.
Consistent tip geometry improves precision when a tool approaches or interacts with a cell or subcellular structure. In microinjection, it supports controlled fluid delivery; in patch-clamp work, it contributes to reliable electrical recording; and in manipulation or sampling, it helps maintain predictable contact with the microscopic target. These benefits connect fabrication quality directly to experimental outcomes.