Cd11c High Cells
Cd11c High Cells - Related Videos
03:03
Identification and Enumeration of Immune Cells in Bronchoalveolar Lavage Fluid by Flow Cytometry
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2025
This video demonstrates the use of flow cytometry to identify and count various immune cells in bronchoalveolar lavage fluid. Initially, single immune cells are identified using a viability dye, followed by differential staining of surface marker proteins, enabling the precise enumeration of each cell type.
07:03
Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration
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Cited by 155 •
2017
The health status of the lung is reflected by the type and number of immune cells that are present in the bronchioles of the lung. We describe a bronchoalveolar lavage technique that allows the isolation and study of nonadherent cells and soluble factors from the lower respiratory tract of mice.
04:39
In Vitro Generation of Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors
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2025
This video demonstrates an in vitro assay to generate plasmacytoid dendritic cells (pDCs) from common lymphoid progenitor cells (CLPs). Upon co-culturing purified CLPs with γ-irradiated feeder cells in the presence of Fms-like tyrosine kinase-3 ligand (FL), the CLPs differentiate into non-adherent pDCs, the presence of which is confirmed using flow cytometry.
08:31
Characterization of Human Monocyte-derived Dendritic Cells by Imaging Flow Cytometry: A Comparison between Two Monocyte Isolation Protocols
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Cited by 19 •
2016
This study compares two different methods of human monocyte isolation for obtaining in vitro dendritic cells (DCs). Monocytes are selected by adherence or negatively enriched by magnetic separation. Monocyte yield and viability along with MDDC viability, proliferation and CD11c/CD14 surface marker expression will be compared between both methods.
03:16
A Method for the High Salt Treatment of Dendritic Cells and their Adoptive Transfer into Mice
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2025
This video demonstrates a procedure for the adoptive transfer of high salt-treated antigen-presenting dendritic cells (DCs) into mice. Murine CD11c+ dendritic cells (DCs) are exposed to a medium containing high amounts of sodium, forming isolevuglandin (IsoLG)-protein adducts in the cells. These adducts serve as neoantigens and are presented on the surface of DCs. The high salt-treated cells are injected into mice through adoptive cell transfer.
FAQs
CD11c pairs with CD18 to form an adhesion receptor that can interact with molecules such as ICAM-1. These interactions support cell attachment, migration, and contact with other immune cells. Consequently, elevated CD11c expression can provide information about immune-cell behavior in tissues, not merely serve as a label for a particular cell population.
CD11c expression is influenced by tissue context and cellular activation, so the intensity observed in one sample may not have the same meaning in another. This variability matters when interpreting CD11c-high populations because changes in expression can reflect altered immune states or local conditions rather than a fixed cellular identity.
No. Dendritic cells commonly show this phenotype, but selected monocyte and macrophage populations can also express elevated CD11c. Therefore, CD11c intensity helps distinguish immune-cell subsets but does not by itself establish that every CD11c-high cell is a dendritic cell. Interpretation should account for the tissue and immune context being studied.
The phenotype can be used to examine how immune-cell populations participate in antigen presentation and inflammation. Because CD11c expression may change with activation, comparing CD11c-high populations across biological conditions can reveal shifts associated with immune responses. This makes the measurement useful for connecting cellular composition with broader inflammatory or immunological changes.
In flow cytometry, researchers measure CD11c expression levels across immune cells and identify the population showing relatively high intensity. This approach supports separation of CD11c-high cells from other immune-cell subsets within the analyzed sample. Comparing the size or intensity of this population between conditions can help track changes during an immune response or disease model.
Tissue analysis evaluates where CD11c-expressing populations occur and how their representation or expression differs between samples. When interpreted with tissue context, these findings can help investigate immune-cell involvement in inflammation and antigen presentation. The approach is especially useful when researchers want to examine immune changes in a biological setting rather than only in an isolated cell measurement.
Researchers use CD11c-high cells to monitor changes in immune populations during disease models and immune responses. Measurements can indicate shifts in the abundance or expression of relevant dendritic, monocyte, or macrophage populations. Combining flow-cytometric or tissue-based observations with the experimental context helps relate those shifts to inflammation and other immune processes.