Cd34 Antibody
Cd34 Antibody - Related Videos
13:21
Antibody Generation: Producing Monoclonal Antibodies Using Hybridomas
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2023
Source: Frances V. Sjaastad1,2, Whitney Swanson2,3, and Thomas S. Griffith1,2,3,4 1 Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, Minneapolis, MN 55455 2 Center for Immunology, University of Minnesota, Minneapolis, MN 55455 3 Department of Urology, University of Minnesota, Minneapolis, MN 55455 4 Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455 Polyclonal antibodies are defined as a collection of antibodies directed against...
01:10
Antibody Structure
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2019
Overview Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding. The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains Antibodies consist of four polypeptide chains: two identical heavy...
12:55
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
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Cited by 33 •
2015
A method is described with visual accompaniment for conducting scalable, high throughput selections from phage-displayed combinatorial synthetic antibody libraries against hundreds of antigens simultaneously. Using this parallel approach, we have isolated antibody fragments that exhibit high affinity and specificity for diverse antigens that are functional in standard immunoassays.
08:14
Immunomagnetic Isolation of the Vascular Wall-Resident CD34+ Stem Cells from Mice
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Cited by 1 •
2023
This study has established a stable and efficient method for the isolation, culture, and functional determination of vascular wall-resident CD34+ stem cells (CD34+ VW-SCs). This easy-to-follow and time-effective isolation method can be utilized by other investigators to study the potential mechanisms involved in cardiovascular diseases.
03:18
Antibody-Dependent Enhancement of Zika Virus by Dengue Antibodies in Human Primary Macrophages
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2026
Source: Asad, S., et al. Quantification of Antibody-dependent Enhancement of the Zika Virus in Primary Human Cells. J. Vis. Exp. (2019)This video demonstrates antibody-dependent enhancement of Zika virus infection in human primary macrophages, using dengue antibody–virus complexes to model how cross-reactive antibodies facilitate Fcγ receptor–mediated viral entry and replication for studying infection mechanisms and immune interactions.
FAQs
Binding depends on whether the antibody recognizes an accessible CD34 epitope on the prepared sample. Different antibody clones may target different epitopes, so they can produce different labeling patterns or measured levels even when used to examine related material. Clone selection therefore matters when comparing hematopoietic stem and progenitor cell populations across experiments or detection platforms.
CD34 is associated with hematopoietic stem and progenitor cells, but it is also expressed by certain endothelial and other cell populations. A positive signal therefore identifies CD34-bearing cells rather than establishing one unique lineage by itself. Interpretation should account for the biological source and the purpose of the analysis, especially when assessing tissue samples.
Antibody clone, sample preparation, and detection method can all influence the observed result. Preparation may affect whether the relevant epitope remains available, while the selected platform determines how labeling is detected, measured, or used for cell isolation. Keeping these variables consistent is important when comparing samples or evaluating changes in CD34-positive populations.
In flow cytometry, the antibody labels CD34-bearing cells so their presence or measured representation can be assessed within a sample. This supports characterization of hematopoietic stem and progenitor cell populations in blood or other biological material. The resulting interpretation still depends on antibody clone and sample preparation, because both can influence the detected signal.
Immunohistochemistry is useful when the goal is to examine CD34-associated cells within tissue context rather than only measure or isolate cells from a sample. A CD34 antibody can reveal labeling in tissue sections and support studies of endothelial or other CD34-expressing populations. This approach contributes to investigations of vascular biology and tissue organization.
Antibody-based magnetic separation uses CD34 recognition to enrich cells bearing the target marker from a mixed sample. The enriched material can support research on hematopoiesis, transplantation, or regenerative medicine by providing a population selected for CD34 association. Because enrichment depends on the antibody and starting sample, the separated fraction should be interpreted as CD34-associated rather than automatically uniform.