Ciliates

Ciliates are single-celled eukaryotes distinguished by coordinated cilia, which drive movement and direct food toward specialized cell surfaces. They maintain distinct nuclear compartments: a transcriptionally active macronucleus supports everyday functions, while a micronucleus carries genetic material for reproduction and inheritance. During feeding, cilia create water currents that sweep bacteria or other particles into an oral groove, where ingestion and digestion occur; during conjugation, cells exchange micronuclear DNA. Ciliates are important grazers in aquatic and soil ecosystems and serve as accessible models for studying cell motility, cytoskeletal organization, membrane trafficking, and programmed cellular processes.

Ciliates - Related Videos

Research

JoVE Journal - Chemistry

Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos

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Cited by 3 •

2016

Techniques are described to immunostain phospho-epitopes in whole zebrafish embryos and then conduct two-color fluorescent confocal localization in cellular structures as small as primary cilia. The techniques for fixing and imaging can define the location and kinetics of the appearance or activation of specific proteins.

Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection

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Cited by 11 •

2017

We explored a tubal cytologic method by sampling the fallopian tube directly post-surgical excision as a tool of ovarian cancer early detection. Here, we present a protocol to collect fallopian tube cells from freshly received surgical specimens.

Papanicolaou Staining: A Technique to Visualize the Cytological Features of Tubal Cells

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2025

In this video, we demonstrate a modified protocol of Papanicolaou staining to study tubal cytology. This technique can be used to visualize the cytological features of malignant and non-malignant fallopian tube epithelial cells.

Ex vivo Method for High Resolution Imaging of Cilia Motility in Rodent Airway Epithelia

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Cited by 49 •

2013

An easy and reliable technique for visualizing and quantifying airway cilia motility and cilia generated flow using mouse trachea is described. This technique can be modified to determine how a wide range of factors influence cilia motility, including pharmacological agents, genetic factors, environmental exposures, and/or mechanical factors such as mucus load.

Research

JoVE Journal - Biology
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Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells

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Cited by 29 •

2011

The fallopian tube (FT) is emerging as an alternative site of origin for serous ovarian carcinoma (SOC). This protocol describes a novel method for the isolation and ex vivo culture of fallopian tube epithelial cells. This system recapitulates the in vivo epithelium and allows the study of SOC pathogenesis.

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