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Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos
 

Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos

Article doi: 10.3791/53747
February 19th, 2016

Summary February 19th, 2016

Techniques are described to immunostain phospho-epitopes in whole zebrafish embryos and then conduct two-color fluorescent confocal localization in cellular structures as small as primary cilia. The techniques for fixing and imaging can define the location and kinetics of the appearance or activation of specific proteins.

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