Collagen Iv
Collagen Iv - Related Videos
01:05
Type IV Collagen of Basal Lamina
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2023
Type IV collagen is a 400 nm long, network-forming collagen that acts as a barrier between the epithelial and endothelial cells. Type IV collagen forms the backbone of the basement membrane by scaffolding with laminin, entactin, proteoglycans, and fibronectin. Apart from rendering structural support to the basement membrane, it also helps entail signaling potentials necessary for both pathological and physiological functions. A type IV collagen molecule has six alpha chains which can exist in...
07:25
Collagen Hydrogels
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2023
Collagen is another widely used biomaterial that has found popularity in commercial applications, such as photography. Collagen has more recently been used in tissue engineering applications, by creating hydrogels that provide structure to engineered tissue. This video introduces collagen as a biomaterial, demonstrates how it is harvested from porcine skin, and shows how the material is used to create a hydrogel for tissue engineering applications. Finally, several applications of the material...
07:03
Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides
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Cited by 18 •
2014
This procedure demonstrates in vivo near IR fluorescence imaging of collagen remodeling activities in mice as well as ex vivo staining of collagens in tissue sections using caged collagen mimetic peptides that can be photo-triggered to hybridize with denatured collagen strands.
05:17
An Improved Method for the Preparation of Type I Collagen From Skin
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Cited by 23 •
2014
Traditional procedures for the isolation of soluble type 1 collagen (COL1) require about 10 days from start to finish because of lengthy buffer incubations and laborious resuspensions of fibrils. Here, we describe a means to purify COL1 from small dermal biopsies in less than 3 hr.
12:08
Improving IV Insulin Administration in a Community Hospital
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Cited by 7 •
2012
When compared to the previous paper protocol, implementation of a computerized glucose management system results in a substantial increase in blood glucose concentration measurements within the target range. Using a computerized glucose management system to monitor blood glucose levels, decreases in severe hypoglycemia (<40 mg/dL), clinical hypoglycemia (<70 mg/dL), and hyperglycemia (>180 mg/dL) also can be observed.
FAQs
Assembly depends on the coordinated organization of alpha chains into triple-helical domains and specialized N- and C-terminal regions. These terminal regions help connect collagen IV molecules into a continuous, sheet-like mesh rather than separate fibers. This architecture allows basement membranes to remain flexible while maintaining structural continuity around epithelial and endothelial cells.
Collagen IV forms an interconnected network instead of the rope-like fibrils associated with fibrillar collagens. Its molecular arrangement produces a mesh that can spread through basement membranes and interact with other extracellular-matrix components. This difference supports specialized functions, including flexible cellular support and selective filtration, rather than primarily providing bundled tensile reinforcement.
Collagen IV does not act as an isolated structural material. Its network interacts with laminin, proteoglycans, and cell-surface receptors, linking extracellular structure with cell attachment and signaling. These connections help cells interpret their surrounding matrix, influence how they adhere to basement membranes, and coordinate tissue organization with mechanical support.
In kidney basement membranes, the collagen IV network contributes to the structural environment through which filtration occurs. Its sheet-like organization provides support while participating in the specialized extracellular matrix associated with kidney function. Studying changes in this network can therefore help connect basement-membrane architecture with altered filtration and broader renal dysfunction.
Research commonly focuses on tissues containing basement membranes that support epithelial, endothelial, and other cells. The kidney is especially informative because basement-membrane organization relates to selective filtration, while vascular tissues provide context for endothelial support. Examining these settings helps reveal how extracellular structure contributes to tissue organization and function.
Changes in collagen IV can disrupt the organization or performance of basement membranes, providing a link between extracellular-matrix structure and disease. Investigators can examine how altered networks affect cellular adhesion, signaling, tissue support, vascular function, or kidney filtration. This approach helps explain disorders that arise when basement-membrane architecture no longer operates normally.