Janus Kinase
Janus Kinase - Related Videos
09:09
Synthesis of Poly(N-isopropylacrylamide) Janus Microhydrogels for Anisotropic Thermo-responsiveness and Organophilic/Hydrophilic Loading Capability
0 Views •
Cited by 3 •
2016
We present a protocol to synthesize Janus microhydrogels composed entirely of the same base material, poly(N-isopropylacrylamide) (PNIPAAm), with a clearly compartmentalized structure base on the phase separation of a supersaturated NIPAAm monomer solution. The synthesized Janus microhydrogels show unique properties such as anisotropic thermo-responsiveness and organophilic/hydrophilic loading capability.
02:54
Protein Kinases and Phosphatases
0 Views •
2020
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven. Protein kinases Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
09:55
Preparation of Janus Particles and Alternating Current Electrokinetic Measurements with a Rapidly Fabricated Indium Tin Oxide Electrode Array
0 Views •
Cited by 2 •
2017
In this article, a simple method to prepare partially or fully coated metallic particles and to perform AC electrokinetic property measurements with a rapidly fabricated indium tin oxide (ITO) electrode array is demonstrated.
06:09
Assaying the Kinase Activity of LRRK2 in vitro
0 Views •
Cited by 3 •
2012
Leucine Rich Repeat Kinase 2 is a large multidomain kinase, mutations in which are the most common genetic cause of Parkinson's disease. Analysis of the kinase activity of this protein has proven to be a crucial tool in understanding the biology and dysfunction of this protein. In this paper, in vitro assaying of the kinase activity of LRRK2 and a selection of its mutants is described, providing an experimental system to examine phosphorylation of putative substrates and potential dysfunction...
11:13
Identification of Kinase-substrate Pairs Using High Throughput Screening
0 Views •
Cited by 1 •
2015
Protein phosphorylation is a central feature of how cells interpret and respond to information in their extracellular milieu. Here, we present a high throughput screening protocol using kinases purified from mammalian cells to rapidly identify kinases that phosphorylate a substrate(s) of interest.
FAQs
Ligand binding brings the associated receptor components into an arrangement that enables their JAKs to activate one another through phosphorylation. The activated kinases then phosphorylate STAT proteins, creating the molecular link between receptor stimulation and altered gene expression. This sequence explains how an extracellular cytokine or hormone signal can produce a specific intracellular response.
Once phosphorylated by JAKs, STAT proteins dimerize, meaning two STAT molecules pair together. The resulting dimers move into the nucleus, where they regulate target genes. This step converts a phosphorylation event at the receptor into transcriptional control, allowing the original signal to influence cellular behaviors such as survival, proliferation, differentiation, or inflammatory activity.
JAK-STAT signaling can regulate cell proliferation, differentiation, and survival, while also shaping inflammatory responses. These outcomes depend on the genes affected after STAT dimers enter the nucleus. In biology research, examining this pathway therefore connects receptor stimulation with both long-term changes in cell behavior and broader immune-system activity.
Because JAK signaling controls gene expression linked to immune responses, proliferation, differentiation, and survival, abnormal pathway activity can disturb several aspects of cell regulation at once. The overview identifies immune disorders and cancer as consequences associated with dysregulated signaling. This connection makes pathway control important for understanding disease mechanisms and developing targeted interventions.
JAKs provide a mechanistic framework for studying how cytokine and hormone receptors communicate with the genome. Researchers can follow the sequence from ligand binding and kinase phosphorylation through STAT activation and nuclear gene regulation, then relate those events to inflammatory or immune outcomes. This helps connect molecular signaling with the behavior of immune-regulating cells.
Selective JAK inhibitors are designed to modulate excessive inflammation by targeting components of the signaling system rather than treating inflammatory activity as an unrelated symptom. Their development follows from the pathway's role in transmitting cytokine signals to gene expression. Studying JAK signaling therefore supports efforts to create targeted treatments for conditions involving excessive immune activation.