Colorectal Tumors
Colorectal Tumors - Related Videos
06:19
Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer
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Cited by 13 •
2017
We describe the establishment of orthotopic colorectal tumors via injection of tumor cells or organoids into the cecum of mice and the subsequent isolation of circulating tumor cells (CTCs) from this model.
08:43
Orthotopic Mouse Model of Colorectal Cancer
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Cited by 109 •
2007
Two techniques can be used to establish this model: injection of a cancer cell suspension into the cecal wall or transplantation of a piece of subcutaneous tumor onto the cecum. This model is useful for studying the natural progression of colorectal cancer and testing new therapeutic agents against colorectal cancer.
10:33
Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
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Cited by 2 •
2014
Molecular profiling of laser microdissected cells and stroma from synthetic, tissue-engineered colorectal cancer models represents a novel and manipulatable approach to characterize and dissect the distinctive biology at the interface between tumor cells at the invasive front and cancer associated stromal cells.
03:19
Generating PDOX Mouse Model of Colorectal Cancer: Studying Progression of Colorectal Cancer in a Murine Model
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2023
This video describes the protocol for generating a patient-derived orthotopic xenograft colorectal cancer mouse model to study the progression of colorectal cancer cells in non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. This murine model helps in understanding primary tumor growth and metastases pattern.
04:08
Intracecal Tumor Cell Injection: A Technique to Deliver Cancer Cells for Establishing Orthotopic Colorectal Cancer Mouse Model
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2023
The video describes the technique for intracecal injection of cancerous cells to develop an orthotopic mouse model with highly uniform tumors. Therefore, the orthotopic mouse model allows realistic simulation of metastasis, providing a valuable tool to study the phenotype changes during tumor growth and dissemination.
FAQs
At the cellular level, genetic and regulatory changes can disturb the balance among intestinal cell proliferation, differentiation, and programmed cell death. When these processes no longer remain coordinated, abnormal growth may persist instead of being limited by normal tissue controls. This imbalance provides a biological basis for investigating how colorectal tumor development begins and progresses.
Some adenomas can acquire additional alterations after their initial development. These changes may support more aggressive behavior, including invasion into surrounding tissue and metastasis to other sites. Studying this progression helps researchers connect successive molecular changes with increasingly advanced tumor behavior, rather than treating all colorectal growths as biologically equivalent.
Colorectal tumor behavior is studied within the context of tissue organization, immune-system interactions, and the surrounding microenvironment. These factors provide biological context for how abnormal cells relate to neighboring structures and host responses. Examining them can clarify why tumor progression differs across biological settings and can reveal processes relevant to disease classification and treatment development.
Researchers examine colorectal tumors by relating altered cell regulation to tissue organization, tumor progression, immune interactions, and the surrounding microenvironment. They also use experimental models to investigate these features and test treatment responses. This combined approach connects biological mechanisms with observable tumor behavior, supporting more informative studies of progression and potential therapeutic strategies.
Research on colorectal tumors can identify biological features associated with abnormal growth and progression. These findings support efforts toward earlier detection and improved disease classification by distinguishing relevant patterns in tumor behavior and regulation. Better classification can help researchers organize disease states more precisely and evaluate which experimental treatment responses correspond to particular tumor characteristics.
Experimental models provide systems for examining colorectal tumor biology and testing treatment responses under research conditions. They can help investigators study how targeted therapies relate to the genetic, regulatory, cellular, and microenvironmental features of tumors. In this way, models connect mechanistic biology with therapeutic development while supporting the evaluation of approaches designed for more specific tumor characteristics.