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Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer
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Cancer Research
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JoVE Journal Cancer Research
Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer

Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer

DOI:
10.3791/55357-v

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06:19 min

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July 18, 2017

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, , , , , ,
1Department of Gastrointestinal, Thoracic and Vascular Surgery, Medizinische Fakultät Carl Gustav Carus,Technische Universität Dresden, 2German Cancer Consortium (DKTK), 3German Cancer Research Center (DKFZ)

Chapters

  • 00:05Title
  • 00:40Orthotopic Mouse Model
  • 03:05Circulating Tumor Cell (CTC) Isolation from Whole Blood
  • 04:59Results: Representative Murine Colorectal Cancer Induction
  • 05:33Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the establishment of orthotopic colorectal tumors via injection of tumor cells or organoids into the cecum of mice and the subsequent isolation of circulating tumor cells (CTCs) from this model.

Tags

Keywords: Circulating Tumor CellsOrthotopic Mouse ModelColorectal CancerMicrosurgical TechniqueCecal Wall InjectionReproducible Metastatic TumorsImmunocompromised MouseIntra-cecal InjectionMicro Injection PumpBinocular Surgical MicroscopeDensity Gradient MediumBlood Sample Collection

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