Crispr Genome Integration
Crispr Genome Integration - Related Videos
02:58
CRISPR-Mediated Base Editing Tools: A Genome Editing Technique to Induce Targeted Base Substitution
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2025
This video explains the concept of CRISPR-mediated cytosine base editors for inducing targeted nucleotide substitution.
03:48
Screening for Genomic Modifications: A Method to Identify CRISPR-Generated Mutants in Drosophila
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2023
This video describes a screening method to identify transgenic Drosophila flies, whose genome was modified using CRISPR-Cas9. CRISPR-Cas9 is a powerful genome-editing tool that revolutionized the way scientists can manipulate an organism's genome. In the example protocol, we will see how to identify CRISPR-generated mutants, in which an insertion of a transactivation sequence replaces the first exon of the branchless (bnl) gene, thus creating a bnl gene-specific driver line, bnl-LexA.
05:12
All-in-One CRISPR Genome Editing: A Method for Homology Directed Repair-Based Gene Knock-In in Cultured Cells Using CRISPR-Cas9 System
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2025
In this video, we demonstrate all-in-one CRISPR-Cas9 based genome editing in cultured cells where Cas9 and sgRNA are provided as a single plasmid construct to the cells. The CRISPR-Cas9 system and desired gene to be inserted was introduced in cells through electroporation technique to facilitate successful gene editing.
02:53
CRISPR and crRNAs
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2020
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea. The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
01:28
CRISPR/Cas9 Genome Editing
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2025
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
FAQs
The guide RNA supplies sequence-specific targeting, while the CRISPR-associated nuclease performs DNA cleavage at the matching site. That cut is important because it activates a cellular repair response rather than merely placing DNA near the genome. In an integration experiment, this division of roles links recognition by the guide RNA to insertion through the cell’s own repair machinery.
A donor template carries the sequence intended for insertion plus flanking regions that match the DNA surrounding the cleavage site. These matching sequences support homology-directed repair, allowing the cell to use the donor while repairing the break. Their role is central to directing the new sequence to the selected genomic location instead of relying on uncontrolled placement.
Targeted CRISPR genome integration differs from random insertion because the experiment specifies where the added sequence should be incorporated. Site control helps researchers interpret the resulting genetic change in relation to the chosen locus and can reduce effects caused by unpredictable insertion sites. This distinction is especially important when studying gene function or creating engineered cells and organisms.
A conceptual workflow begins by selecting a genomic location, designing a guide RNA that matches nearby DNA, and preparing a donor template with compatible flanking sequences. The nuclease then cleaves the selected site, and cellular homology-directed repair uses the donor during repair. This sequence connects experimental design, targeted cleavage, and stable incorporation into one genome-engineering strategy.
Researchers can use the approach to add reporter sequences, create disease models, or modify a locus for functional genomics. Reporter integration places a designed sequence at a selected genomic site, while disease modeling introduces a defined genetic change for biological study. The same targeting principle also supports the development of engineered cells and organisms.
The method is useful when the location of a genetic change matters as much as the sequence being added. A defined integration site helps relate engineered DNA to gene function, disease modeling, or a designed trait, while stable incorporation allows researchers to examine the resulting engineered cells or organisms during subsequent biological investigations.