Airborne Particle Assessment
Airborne Particle Assessment - Related Videos
09:29
An Air-liquid Interface Bronchial Epithelial Model for Realistic, Repeated Inhalation Exposure to Airborne Particles for Toxicity Testing
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Cited by 45 •
2020
Described is the cell culture and exposure method of an in vitro bronchial model for realistic, repeated inhalation exposure to particles for toxicity testing.
10:10
Assessment of the Acute Inhalation Toxicity of Airborne Particles by Exposing Cultivated Human Lung Cells at the Air-Liquid Interface
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Cited by 2 •
2020
We present a robust, transferable and predictive in vitro exposure system for the screening and monitoring of airborne particles concerning their acute pulmonary cytotoxicity by exposing cultivated human lung cells at the air-liquid interface (ALI).
10:42
Flow Cytometric Analysis of Particle-bound Bet v 1 Allergen in PM10
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Cited by 8 •
2016
Here, we present a protocol to quantify allergen-loaded particles by flow cytometry. Ambient particulate matter particles may act as carriers of adsorbed allergens. We show here that flow cytometry, a method widely used to characterize suspended solids >0.5 µm in diameter, can be used to measure these allergen-loaded particles.
01:25
Transmission-based Precautions II: Airborne and Protective Environment
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2023
Transmission-based precautions are for patients infected or suspected to be infected (or colonized) with organisms posing a significant risk to others. The transmission precautions include airborne and protective environment precautions. Airborne precautions: Use airborne precautions when treating patients known or suspected to have diseases that spread through the air—for example, tuberculosis or measles. These organisms are present in smaller droplets expelled by an infected person and...
03:37
Subatomic Particles
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2020
Dalton was only partially correct about the particles that make up matter. All matter is composed of atoms, and atoms are composed of three smaller subatomic particles: protons, neutrons, and electrons. These three particles account for the mass and the charge of an atom. The Discovery of the Electron The first clue about the subatomic structure came at the end of the 19th century when J.J. Thomson discovered the electron using a cathode ray tube. This apparatus consisted of a sealed glass...
FAQs
These measurements describe different aspects of an airborne population. Concentration indicates how many particles are present in a sampled air volume, size distribution shows how particles are apportioned across sizes, and mass reflects their total collected weight. Considering them together gives a more informative picture of environmental conditions and exposure than relying on one measurement alone.
The volume drawn through the sampling device provides the reference needed to interpret particle counts or collected material. Without a defined air volume, results cannot be meaningfully expressed as a concentration or compared reliably between samples. Consistent volume measurement therefore supports comparisons of environmental conditions, exposure, and biological aerosol movement across locations or sampling periods.
Size distribution helps describe the range of biological particles in an airborne sample, including microbial cells, spores, pollen, and respiratory droplets. It can also add context to studies of inhalation exposure and airborne transmission. This measurement therefore provides information beyond a single overall particle concentration and helps characterize how biological material is represented in air.
These approaches quantify different observable features. Optical detection supports particle measurement through optical signals, filtration collects particles for subsequent examination, microscopy supports particle characterization, and gravimetric analysis estimates mass from collected material. The appropriate method depends on whether the study emphasizes concentration, particle characteristics, or mass, and the methods can provide complementary information.
A useful workflow should specify the air volume to be drawn, the sampling device, and the measurement target, such as concentration, size distribution, or mass. It should also identify whether the sample will be evaluated by optical detection, filtration, microscopy, or gravimetric analysis. These choices align collection with the biological question and make results interpretable.
It is useful when researchers need to examine bioaerosols, including microbial cells, spores, pollen, or respiratory droplets, in natural or built environments. Applications include investigating airborne transmission, estimating inhalation exposure, evaluating indoor air quality, and studying contamination control. The resulting measurements also inform biosafety practices and improve understanding of biological particle movement.