Podocyte Marker Expression
Podocyte Marker Expression - Related Videos
04:19
Deriving Human Kidney Podocytes from Induced Pluripotent Stem Cells: A Procedure for Directed Differentiation of Mature Kidney Podocytes from Stem Cells
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2023
In this video, we describe the protocol to derive human kidney podocytes from induced pluripotent stem cells through cell differentiation under defined chemical conditions. The generated podocytes can be used for nephrotoxicity testing and disease modeling.
12:19
Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli
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Cited by 25 •
2015
Changes in the intracellular calcium levels in the podocytes are one of the most important means to control the filtration function of glomeruli. Here we explain a high-throughput approach that allows detection of real-time calcium handling and single ion channels activity in the podocytes of the freshly isolated glomeruli.
06:56
Near Infrared (NIr) Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium
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Cited by 4 •
2015
Mitochondrial dysfunction is a hallmark of cellular senescence. This paper uses non-invasive near-infrared (NIr) treatment to improve mitochondrial function in the aging mouse vestibular sensory epithelium.
03:19
Transduction to Label PDX Tumor Cells: Introducing Lentivirus Expressing Fluorescent Marker into the Tumor Cell In Vitro
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2023
The following video describes a technique of transduction to label (patient-derived xenografts) PDX tumor cells, which is used for introducing lentivirus expressing green-fluorescent protein and luciferase reporters into the tumor cell in vitro.
05:30
Trophoblast Cell Recovery from Angiogenesis-Tube Formation Assay for Differentiation Marker Expression Analysis
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Cited by 3 •
2024
The present protocol allows the recovery of tube-like structures formed in the classical in vitro angiogenesis-tube formation assay where cells, such as trophoblast cells, organize in capillary-like structures over a layer of extracellular basement membrane matrix. The recovered structures are used for RNA and protein isolation for various biochemical analyses.
FAQs
Abundance and localization provide complementary evidence about podocyte status. Reduced marker levels may accompany injury, dedifferentiation, or loss, while altered localization can indicate disruption of specialized podocyte structure. Interpreting these patterns alongside slit diaphragm and cytoskeletal changes helps researchers determine whether a model reflects compromised podocyte organization, altered identity, or reduced podocyte presence.
These markers reflect different aspects of podocyte identity and specialization, so they do not provide identical information. Nephrin and podocin are associated with the slit diaphragm, synaptopodin reflects specialized structure, and WT1 supports assessment of podocyte identity. Examining several markers together gives a broader view of differentiation and structural integrity than relying on one signal alone.
Marker patterns can reveal transitions in podocyte state, including differentiation during glomerular development and injury-associated dedifferentiation or loss. Changes may occur with disruption of the slit diaphragm and cytoskeleton, linking molecular measurements to filtration-barrier organization. This makes expression analysis useful for connecting cellular phenotype with changes relevant to proteinuric disease models.
The appropriate method depends on whether the study needs localization, protein abundance, or transcript measurement. Immunofluorescence and immunohistochemistry show where marker signals occur, whereas western blotting evaluates protein abundance. RT-qPCR measures marker transcripts. Because these approaches answer different questions, combining them can distinguish altered distribution from changes in total protein or transcript levels.
A typical workflow selects markers that represent podocyte identity and specialized structure, then measures them with immunofluorescence, immunohistochemistry, western blotting, or RT-qPCR. Researchers compare marker abundance or localization across developmental, disease, or treatment conditions. The resulting patterns are interpreted together with slit diaphragm and cytoskeletal status to evaluate podocyte phenotype and filtration-barrier integrity.
These measurements support several research goals: characterizing glomerular development, investigating proteinuric diseases, evaluating experimental models, and assessing therapies intended to preserve filtration function. Marker results can show whether a model exhibits podocyte differentiation, injury, dedifferentiation, or loss, while localization and structural findings help relate molecular changes to the condition of the glomerular filtration barrier.