Cryopreservation Method
Cryopreservation Method - Related Videos
11:01
A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
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Cited by 29 •
2009
This is a high-throughput sperm cryopreservation protocol for zebrafish. Sperm cryopreserved using this protocol has an average of 25% fertility in subsequent vitro fertilization and is stable over many years.
07:06
Mouse Sperm Cryopreservation and Recovery using the I·Cryo Kit
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Cited by 5 •
2011
Here we demonstrate the newly developed I•Cryo kit for mouse sperm cryopreservation. Two-cell stage embryo development with frozen-thawed sperm was improved consistently in 5 mouse strains with the use of this kit. Over a 1.5 year period, 49 genetically modified mouse lines were archived by sperm cryopreservation with the I•Cryo kit and later successfully recovered by IVF.
07:05
Isolation and Cryopreservation of Neonatal Rat Cardiomyocytes
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Cited by 49 •
2015
The isolation of neonatal rat cardiomyocytes is a time consuming and unpredictable procedure. This study describes methods for cryopreservation and thawing of neonatal rat cardiomyocytes that allows for more efficient use of cells. The thawed NRCMs can be used for various experiments without the need for performing isolations each time.
11:10
Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
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Cited by 23 •
2011
Preimplantation embryos may be cryopreserved after placement into a hypertonic cryoprotective solution to cause cellular dehydration. After equilibration, ice crystal formation is induced in the solution surrounding the embryo. Further dehydration occurs as the embryo is slowly cooled to subzero temperatures before plunging into liquid nitrogen for storage.
02:35
Long-Term Cryopreservation of Cyanobacterial Strains Using Glycerol or Dimethyl Sulfoxide
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2025
Source: Lea-Smith, D. J., et al. Generation of Marked and Markerless Mutants in Model Cyanobacterial Species. J. Vis. Exp. (2016)This video demonstrates the cryopreservation of cyanobacterial strains by combining concentrated cell suspensions with glycerol or DMSO and storing them at low temperatures, enabling long-term maintenance of viable cultures for photosynthesis and genetic studies.
FAQs
Cryoprotective agents reduce ice-crystal formation as temperature falls. This protection is important because uncontrolled crystallization can damage cells, tissues, or other preserved material and reduce recovery of biological function. Their use works together with an appropriate cooling strategy, helping maintain viability during storage and increasing the likelihood that the sample can be successfully recovered later.
Controlled-rate cooling and vitrification are two approaches for preparing biological material for ultra-low-temperature storage. Controlled-rate cooling gradually reduces temperature, whereas vitrification uses a process that forms a glass-like state rather than allowing damaging ice crystals to develop. The distinction matters because the selected approach influences how effectively the sample is protected before storage and warming.
Careful warming is necessary because preservation does not end when the sample reaches storage temperature. The warming process must help restore biological function while minimizing damage during temperature recovery. If warming is poorly controlled, the material may not regain its intended viability, limiting the usefulness of the stored sample for later experiments, banking, reproduction, transplantation research, or conservation.
A typical workflow includes preparing the biological material with cryoprotective agents, applying controlled-rate cooling or vitrification, transferring the material to ultra-low-temperature storage, and later warming it carefully for recovery. Each stage supports the next: protection limits ice damage, the cooling strategy prepares the sample, storage preserves it, and warming aims to restore function with minimal loss.
Researchers use cryopreservation when biological material must remain available beyond the period in which it would otherwise deteriorate. It supports cell banking, long-term storage of experimental samples, assisted reproduction, transplantation research, and conservation of genetic resources. By extending access to valuable material, the method can make experiments more reproducible and reduce dependence on immediate sample availability.
Cryopreservation can preserve valuable cells, tissues, and other biological materials for later recovery and study. This capacity supports research that would be difficult if samples deteriorated before use, including work involving genetic-resource conservation, transplantation, assisted reproduction, and biological repositories. Maintaining material over time also helps researchers repeat experiments and compare results using stored samples.