Synthetic Media Seal

A synthetic media seal is a protective closure used to cover culture vessels containing laboratory-prepared growth media, helping preserve sterility and maintain stable experimental conditions. By forming a barrier between the medium and the surrounding environment, the seal limits entry of microorganisms and particles while reducing evaporation and unwanted changes in concentration; its design may also regulate gas exchange when required by the culture system. Synthetic media seals support reproducible work in microbiology, cell biology, and biotechnology by protecting cultures during incubation, transport, and storage. Proper sealing improves sample integrity and strengthens the reliability of downstream measurements.

Synthetic Media Seal - Related Videos

Education

JoVE Science Education - Engineering

Synthetic Biology

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2023

This video presents synthetic biology and its role in bioengineering. Synthetic biology refers to the methods used to genetically modify organisms in order to make them capable of producing large quantities of a product. This product could be a protein that the cell already makes or a new protein that has been encoded in a newly-inserted DNA sequence. Here, we discuss how an organism's genetic material is modified using transformation or transfection. Then, the process is shown in the...

Research

JoVE Journal - Engineering

Optimized Sealing Process and Real-Time Monitoring of Glass-to-Metal Seal Structures

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Cited by 7 •

2019

Key procedures to optimize the sealing process and achieve real-time monitoring of the metal-to-glass seal (MTGS) structure are described in detail. The embedded fiber Bragg grating (FBG) sensor is designed to achieve online monitoring of temperature and high-level residual stress in the MTGS with simultaneous environmental pressure monitoring.

Research

JoVE Journal - Medicine
Free Sample

The C-seal: A Biofragmentable Drain Protecting the Stapled Colorectal Anastomosis from Leakage

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Cited by 8 •

2010

The C-seal is a biofragmentable drain protecting the stapled colorectal anastomosis. In this video, details of the manufacturing, deployment and use are shown.

Research

JoVE Journal - Biology
Free Sample

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology

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Cited by 380 •

2013

This five-day protocol outlines all steps, equipment, and supplemental software necessary for creating and running an efficient endogenous Escherichia coli based TX-TL cell-free expression system from scratch. With reagents, the protocol takes 8 hours or less to setup a reaction, collect, and process data.

Production of Synthetic Nuclear Melt Glass

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Cited by 1 •

2016

A protocol for the production of synthetic nuclear melt glass, similar to trinitite, is presented.

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