Degassing Process
Degassing Process - Related Videos
08:18
Degassing Liquids with Freeze-Pump-Thaw Cycling
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2023
Source: Laboratory of Dr. Neil Branda — Simon Fraser University Degassing refers to the process by which dissolved gases are removed from a liquid. The presence of dissolved gases such as oxygen or carbon dioxide can impede chemical reactions that utilize sensitive reagents, interfere with spectroscopic measurements, or can induce unwanted bubble formation. A number of different techniques are available for degassing liquids; some of these include heating, ultrasonic agitation, chemical...
10:32
Fabrication Process of Silicone-based Dielectric Elastomer Actuators
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Cited by 102 •
2016
This manuscript shows the fabrication process for the manufacture of dielectric elastomer soft actuators based on silicone membranes. The three key stages of production are presented in detail: blade casting of thin silicone membranes; pad printing of compliant electrodes; and the assembly of all the components.
01:29
Processes of Self-Presentation
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2025
Effective self-presentation is a central component of social interaction and identity construction. It relies on the dynamic processes of defining the situation and engaging in self-disclosure. These mechanisms help individuals navigate social context expectations and manage how others perceive them, fostering mutual understanding and relationship development.Defining the SituationSocial situations are shaped by collectively understood frames—a set of widely understood rules or conventions...
01:20
Parallel Processing
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2024
The brain processes sensory information rapidly due to parallel processing, which involves sending data across multiple neural pathways at the same time. This method allows the brain to manage various sensory qualities, such as shapes, colors, movements, and locations, all concurrently. For instance, when observing a forest landscape, the brain simultaneously processes the movement of leaves, the shapes of trees, the depth between them, and the various shades of green. This enables a quick and...
01:25
Thermodynamic Processes
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2026
A thermodynamic process is a path through a sequence of states that takes a system from an initial state to a final state. In a cyclic process, the system returns to its initial state, so the changes in state properties and state functions (ΔT, Δp, ΔV, ΔU, ΔH) over one complete cycle are zero. However, heat and work transfers can still occur during the cycle, and the net heat and net work over the cycle need not be zero.A reversible process occurs when the system is infinitesimally close to...
FAQs
Reduced pressure lowers the pressure surrounding a liquid, making dissolved gases less soluble. Oxygen and carbon dioxide can then leave the liquid and form bubbles that escape from the material. In biological solutions, this mechanism helps reduce trapped air before pipetting, microscopy, flow-based work, or microfluidic measurements, where bubbles can disrupt handling and observations.
Heating promotes gas release by lowering the amount of gas that remains dissolved in a liquid. As solubility decreases, trapped oxygen or carbon dioxide can escape more readily. This approach is one of several available mechanisms, alongside vacuum and gas displacement, and its use is relevant when the goal is to reduce dissolved gases in media, buffers, or experimental solutions.
Gas displacement changes the surrounding gas environment so that dissolved gases can leave the liquid, whereas reduced pressure directly lowers the pressure acting on the solution. Both approaches can reduce trapped gases, but they provide different ways to influence gas release. In biological experiments, the choice can be relevant when controlling oxygen exposure is important for anaerobic or oxygen-sensitive organisms.
Bubbles can interfere with pipetting, microscopy, flow systems, and microfluidic measurements, creating physical disturbances that reduce experimental consistency. Dissolved gases also influence the chemical and physical behavior of solutions, particularly when oxygen levels affect biological systems. Degassing therefore helps limit gas-related artifacts and supports more reproducible handling and measurement in laboratory workflows.
The method should match the intended gas-control requirement and the material being treated. Reduced pressure, heating, and gas displacement offer different ways to lower dissolved gases or alter oxygen exposure. Researchers may therefore select an approach based on whether the priority is removing trapped air from a solution, controlling oxygen levels, or improving compatibility with a particular experimental system.
Degassing is useful when bubbles or dissolved gases could affect cell culture, biochemical assays, or laboratory instrumentation. It can improve solution handling and reduce artifacts in measurements, while controlled oxygen levels support studies involving anaerobic or oxygen-sensitive organisms. The process is also relevant to microscopy, flow systems, and microfluidic experiments that are easily disrupted by trapped air.
Removing trapped air from culture media, buffers, and experimental solutions can make pipetting and other handling steps more consistent. Lowering unwanted gases also reduces artifacts that might complicate biochemical measurements or cell-culture procedures. When oxygen must be controlled, the same general approach can support experiments focused on anaerobic or oxygen-sensitive organisms and their responses to defined conditions.
In flow systems and microfluidic devices, trapped bubbles can interrupt liquid movement and interfere with measurements or imaging. Treating the working solution to reduce dissolved gases helps limit bubble formation during use. This improves the reliability of fluid handling and observation, making degassing a practical preparation step when biological experiments depend on stable flow or unobstructed microscopy.