Degassing Process

The degassing process removes dissolved gases, such as oxygen and carbon dioxide, from a liquid or material to control its chemical and physical behavior. In biological research, degassing commonly uses reduced pressure, vacuum, heating, or gas displacement to lower gas solubility and release bubbles from culture media, buffers, and experimental solutions. Removing trapped air helps prevent bubbles that interfere with pipetting, microscopy, flow systems, and microfluidic measurements, while controlled oxygen levels can support studies of anaerobic or oxygen-sensitive organisms. Careful degassing improves reproducibility and reduces artifacts in cell culture, biochemical assays, and laboratory instrumentation.

Degassing Process - Related Videos

Education

JoVE Science Education - Chemistry

Degassing Liquids with Freeze-Pump-Thaw Cycling

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2023

Source: Laboratory of Dr. Neil Branda — Simon Fraser University Degassing refers to the process by which dissolved gases are removed from a liquid. The presence of dissolved gases such as oxygen or carbon dioxide can impede chemical reactions that utilize sensitive reagents, interfere with spectroscopic measurements, or can induce unwanted bubble formation. A number of different techniques are available for degassing liquids; some of these include heating, ultrasonic agitation, chemical...

Research

JoVE Journal - Engineering
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Fabrication Process of Silicone-based Dielectric Elastomer Actuators

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Cited by 102 •

2016

This manuscript shows the fabrication process for the manufacture of dielectric elastomer soft actuators based on silicone membranes. The three key stages of production are presented in detail: blade casting of thin silicone membranes; pad printing of compliant electrodes; and the assembly of all the components.

Processes at Electrodes

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2026

The electrode interacts with ions in the electrolyte solution at its interface. The rate of oxidation and reduction depends on the speed at which electrons can transfer through this interface. As ions attach to or leave the electrode surface, the electrode acquires a charge, and an electrical potential forms across the interface, making the process more difficult to reach equilibrium. The charge on the electrode affects the local ion concentrations in the solution, though thermal motion...

Isothermal Processes

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2025

A thermodynamic process that occurs at constant temperature is called an isothermal process. Heat slowly flows into the system or out of the system to maintain thermal equilibrium. Processes involving phase changes like water evaporation into steam or freezing water into ice at a constant temperature are examples of Isothermal Processes. An ideal gas can also undergo isothermal expansion or compression. For example, consider 1 mole of an ideal gas inside an isolated cylinder at initial volume V...

Work Done in an Adiabatic Process

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2025

Consider the adiabatic compression of an ideal gas in the cylinder of an automobile diesel engine. The gasoline vapor is injected into the cylinder of an automobile engine when the piston is in its expanded position. The temperature, pressure, and volume of the resulting gas-air mixture are 20 °C, 1.00 x 105 N/m2, and 240 cm3 , respectively. The mixture is then compressed adiabatically to a volume of 40 cm3. Note that, in the actual operation of an automobile engine, the compression is not...

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