Digital Camera Imaging

Digital camera imaging is the capture and conversion of light into digital data, enabling biological specimens, structures, and processes to be recorded, measured, and analyzed. In a camera, incoming light reaches a photosensitive sensor, where arrays of pixels convert differences in light intensity and color into electrical signals that software stores as an image. In biology, this method supports microscopy, specimen documentation, time-lapse observation, and quantitative analysis of features such as shape, size, location, and movement. Careful control of illumination, focus, exposure, and image resolution improves data quality and strengthens comparisons across samples and experiments.

Digital Camera Imaging - Related Videos

Research

JoVE Journal - Engineering
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Determining 3D Flow Fields via Multi-camera Light Field Imaging

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Cited by 7 •

2013

A technique for performing quantitative three-dimensional (3D) imaging for a range of fluid flows is presented. Using concepts from the area of Light Field Imaging, we reconstruct 3D volumes from arrays of images. Our 3D results span a broad range including velocity fields and multi-phase bubble size distributions.

Research

JoVE Journal - Bioengineering

Simultaneously Capturing Real-time Images in Two Emission Channels Using a Dual Camera Emission Splitting System: Applications to Cell Adhesion

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Cited by 3 •

2013

Dual camera emission splitting systems for two-color fluorescence microscopy generate real-time image sequences with exceptional optical and temporal resolution, a requirement of certain live cell assays including parallel plate flow chamber adhesion assays. When software is employed to merge images from simultaneously acquired emission channels, pseudocolored image sequences are produced.

Research

JoVE Journal - Engineering
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High-resolution, High-speed, Three-dimensional Video Imaging with Digital Fringe Projection Techniques

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Cited by 9 •

2013

This video describes the fundamentals of digital fringe projection techniques, which provide dense 3D measurements of dynamically changing surfaces. It also demonstrates the design and operation of a high-speed binary defocusing system based on these techniques.

Shrinkage of Dental Composite in Simulated Cavity Measured with Digital Image Correlation

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Cited by 12 •

2014

In order to understand the spatial development of polymerization shrinkage stress in dental resin-composite restorations, Digital Image Correlation was used to provide full-field displacement/strain measurement of restored model glass cavities by correlating images of the restoration taken before and after polymerization.

Imaging and Quantification of the Area of Fast-Moving Microbubbles Using a High-Speed Camera and Image Analysis

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Cited by 4 •

2020

Cavitation microbubbles are imaged using a high-speed camera attached to a zoom lens. The experimental setup is explained, and image analysis is used to calculate the area of the cavitation. Image analysis is done using ImageJ.

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