Dna Transcription
Dna Transcription - Related Videos
05:00
Fluorescence Anisotropy to Determine Transcription Factor-DNA Binding Affinity
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2025
This video describes high-performance fluorescence anisotropy that helps to monitor the interaction between transcription factors and DNA. The assay monitors the interactions of a fluorophore-labeled DNA molecule with its specific transcription factor by measuring the degree of polarization due to molecular rotation or anisotropy of the fluorophore-labeled DNA.
04:31
Electrophoretic Mobility Shift Assay for Detection of Transcription Factor-DNA Complexes
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2025
In this video, we demonstrate the electrophoretic mobility shift assay to detect protein-DNA interactions using infrared fluorescent-labeled DNA probes. When the protein binds to the DNA probe, it forms a large complex that migrates slowly on and appears as a shifted band on the gel.
11:19
Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)
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Cited by 9 •
2015
DNA methylation is capable of maintaining stable levels of gene expression as well as allowing for dynamic changes in gene expression in response to a variety of stimuli. We detail techniques that allow the study of gene-specific changes in DNA methylation and the effect of these changes on gene expression.
02:16
Transcription Factors
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2019
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
02:35
Transcription Elongation Factors
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2020
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene. The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
FAQs
RNA polymerase initiates transcription at a gene’s promoter, a DNA region that marks where copying begins. After binding, the enzyme separates the DNA strands and uses one strand as a template for assembling ribonucleotides. This ordered start point helps connect a particular DNA region with the RNA molecule produced from it, supporting controlled gene expression.
Complementary pairing with the template strand determines the sequence of the growing RNA molecule. As RNA polymerase adds ribonucleotides, the RNA records genetic information in a form that can function in gene expression. The template therefore provides sequence guidance, while the newly made RNA carries a corresponding copy rather than serving as an identical DNA strand.
Termination signals mark where RNA synthesis ends. When RNA polymerase reaches such a signal, production of the transcript stops, creating a defined RNA product rather than an indefinitely extended strand. This endpoint is important because the resulting molecule can then perform its intended role, whether it becomes messenger RNA or contributes to regulation and other cellular functions.
Not every transcript has the same cellular destination. Messenger RNA can guide protein synthesis, whereas other RNA molecules support regulation and cellular function. This distinction broadens transcription beyond a simple route to proteins: the RNA product’s role determines how the copied information contributes to cellular activity.
Transcription supplies the messenger RNA that can guide protein synthesis, linking stored genetic information with protein production. The connection is indirect: DNA information is first represented in an RNA molecule, and that molecule then provides guidance for making a protein. This intermediate step places RNA between DNA information and protein production during gene expression.
Studying transcription helps connect genetic information with cell behavior during development and responses to environmental signals. Researchers can examine the copying step to understand how cells regulate which information enters RNA-based gene expression. This makes transcription relevant to questions about changing cellular states and protein production.
Because transcription influences which genetic information becomes available as RNA, it is relevant to proteins involved in health and disease. Examining messenger RNA and other RNA products can help relate gene activity to cellular function, regulation, and disease-related outcomes. Its study therefore connects molecular events in DNA with broader biological outcomes.