Journal
/
Biology
/
Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)

DOI:
10.3791/52406-v

•

11:19 min

•

September 26, 2015

•

,
1Department of Cell Developmental and Integrative Biology,University of Alabama at Birmingham

Chapters

  • 00:05Title
  • 01:25RNA and DNA Isolation from an Enriched Astrocytic Population using FACS
  • 04:03Assessing DNA Methylation Status of a Gene Using Methylation-sensitive High Resolution Melt Analysis
  • 05:26Assessing Hyper-methylated Promoter Activity using a Luciferase Assay
  • 08:50Results: DNA Methylation Analysis of KCNJ10 in Astrocytes
  • 10:42Conclusion

Summary

Automatic Translation

Automatic Translation

DNA methylation is capable of maintaining stable levels of gene expression as well as allowing for dynamic changes in gene expression in response to a variety of stimuli. We detail techniques that allow the study of gene-specific changes in DNA methylation and the effect of these changes on gene expression.

Tags

Keywords: DNA MethylationGene ExpressionTranscriptional ActivityAstrocytesKCNJ10 (Kir4.1)NeuroepigeneticsFACS SortingMS-HRMALuciferase Promoter AssayNeuropsychiatric DisordersNeurodegenerative DisordersSynaptic PlasticityCNS InjuryTranscriptomeProteomeEpigenome

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52406?article_type=v

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
check_url/52406?article_type=v

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
check_url/52406?article_type=v

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
check_url/52406?article_type=v

Determination of DNA Methylation of Imprinted Genes in Arabidopsis Endosperm
check_url/52406?article_type=v

DNA Methylation: Bisulphite Modification and Analysis
check_url/52406?article_type=v

Single Cell Transcriptional Profiling of Adult Mouse Cardiomyocytes
check_url/52406?article_type=v

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
check_url/52406?article_type=v

A Quantitative Assay to Study Protein:DNA Interactions, Discover Transcriptional Regulators of Gene Expression, and Identify Novel Anti-tumor Agents
check_url/52406?article_type=v

In vitro Methylation Assay to Study Protein Arginine Methylation
check_url/52406?article_type=v

Enhanced Reduced Representation Bisulfite Sequencing for Assessment of DNA Methylation at Base Pair Resolution

Read Article