Donor Recipient Cells
Donor Recipient Cells - Related Videos
12:52
Conjugation: A Method to Transfer Ampicillin Resistance from Donor to Recipient E. coli
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2023
Source: Alexander S. Gold1, Tonya M. Colpitts1 1 Department of Microbiology, Boston University School of Medicine, National Emerging Infections Diseases Laboratories, Boston, MA First discovered by Lederberg and Tatum in 1946, conjugation is a form of horizontal gene transfer between bacteria that relies on direct physical contact between two bacterial cells (1). Unlike other forms of gene transfer, such as transformation or transduction, conjugation is a naturally occurring process in which...
04:05
Subcutaneous Transplantation Assay: A Method to Transplant Melanoma Cells from a Donor to a Recipient Zebrafish Model
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2023
This video describes the method for transplantation of tumor cells from the melanoma-bearing donor zebrafish model to an immunocompromised recipient Casper zebrafish. It helps to visualize tumor engraftment and proliferation in the recipient fish.
11:21
Phage Transduction: A Method to Transfer Ampicillin Resistance from Donor to Recipient E. coli
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2023
Source: Alexander S. Gold1, Tonya M. Colpitts1 1 Department of Microbiology, Boston University School of Medicine, National Emerging Infections Diseases Laboratories, Boston, MA Transduction is a form of genetic exchange between bacteria that utilizes bacteriophages, or phages, a class of virus that infects exclusively prokaryotic organisms. This form of DNA transfer, from one bacterium to another by way of a phage, was discovered in 1951 by Norton Zinder and Joshua Ledererg, who termed the...
03:15
A Peptide Array-Based Detection of Anti-donor HLA Alloantibodies in Organ Recipients
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2025
This video demonstrates an assay to detect anti-donor HLA alloantibodies in organ recipients. A peptide array is synthesized to represent potential epitopes for antibody-mediated organ rejection. The array is incubated with the recipient's serum, containing alloantibodies that bind to their target epitopes. Antibody binding is detected using chemiluminescence to identify donor-specific HLA epitopes that incite alloantibody reactivity.
11:59
Detection of Residual Donor Erythroid Progenitor Cells after Hematopoietic Stem Cell Transplantation for Patients with Hemoglobinopathies
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Cited by 2 •
2017
Quantification of donor-derived cells is required to monitor engraftment after stem cell transplantation in patients with hemoglobinopathies. A combination of flow cytometry-based cell sorting, colony formation assay, and subsequent analysis of short tandem repeats may be used to assess the proliferation and differentiation of progenitors in the erythroid compartment.
FAQs
The recipient’s immune system can identify molecules on donor cell surfaces as foreign, particularly human leukocyte antigens. This recognition activates immune responses directed against the transferred cells, reducing their survival or preventing successful integration. The strength of the response depends on how closely donor and recipient characteristics match, making immune recognition a central biological challenge in transplantation.
Human leukocyte antigens provide surface markers that help immune cells distinguish the body’s own cells from foreign cells. Differences between donor and recipient antigens can increase the likelihood that transferred cells will be targeted. Comparing these characteristics helps researchers and clinicians evaluate compatibility and identify situations in which additional measures may be needed to support cell survival.
Immunosuppressive treatment reduces the recipient’s immune reaction against donor cells, which can improve their survival and opportunity to integrate into the host. Its role is supportive rather than a replacement for compatibility assessment. Because successful transfer also depends on monitoring immune reactions, treatment decisions must be considered alongside donor-recipient comparisons and observed cellular responses.
Researchers compare donor and recipient characteristics, with cellular compatibility receiving particular attention. Surface molecules such as human leukocyte antigens are important because mismatches may promote immune recognition. These comparisons help anticipate rejection-related problems and guide decisions about monitoring or immunosuppressive treatment, improving the ability to evaluate whether transferred cells may persist and integrate.
Monitoring focuses on detecting immune reactions and evaluating whether the transferred cells remain viable and integrate with the host. Researchers use the observed response to determine whether compatibility concerns are producing rejection-related effects and whether supportive immunosuppressive treatment may be needed. This follow-up is essential for assessing outcomes in transplantation and cell-based therapy studies.
Their interactions are studied in bone marrow transplantation, tissue repair, disease modeling, and regenerative medicine. These applications differ in purpose, but each requires understanding whether transferred cells can survive and function within a recipient environment. Findings from compatibility and immune-response studies support efforts to improve integration and develop safer cell-based therapeutic strategies.