Dorsal Brain Isolation
Dorsal Brain Isolation - Related Videos
06:56
Isolation of Macrophages from Mouse Dorsal Root Ganglion Using Mechanical Dissociation
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2025
This video demonstrates an enzyme-free protocol to isolate macrophages from mouse dorsal root ganglion (DRG) using a mechanical dissociation technique. Upon homogenization of the freshly collected DRG tissue, antibodies against the cell-surface receptors on the macrophages are used to label the cells fluorescently, followed by performing flow cytometry to confirm the presence of macrophages in the sample.
08:10
Hydraulic Extrusion of the Spinal Cord and Isolation of Dorsal Root Ganglia in Rodents
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Cited by 110 •
2017
Here, we present a protocol for hydraulic extrusion of the spinal cord as well as identification and isolation of specific dorsal root ganglia (DRGs) in the same rodent. Compared to standard spinal cord isolation methods, this method is significantly faster and reduces the risk of tissue damage.
06:28
Isolating Immune Cells from Mouse Brain and Skull
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Cited by 1 •
2024
To investigate the immune response to brain disorders, one common approach is to analyze changes in immune cells. Here, two simple and effective protocols are provided for isolating immune cells from murine brain tissue and skull bone marrow.
10:24
Mouse Dorsal Forebrain Explant Isolation
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2007
This video demonstrates the protocol for isolating and culturing explants of the mouse forebrain from embyonic day 12 mice. Procedures for removal of the uterus, embryos from uterus, and dissection of embryos are given. In addition the methodology for transferring these explants onto specialized membranes on which they are cultured is demonstrated. The development of the forebrain can be studied in vitro using this preparations as well as changes in gene expression.
06:51
Isolating Hair Follicle Stem Cells and Epidermal Keratinocytes from Dorsal Mouse Skin
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Cited by 23 •
2016
An ideal model for studying adult stem cell biology is the mouse hair follicle. Here we present a protocol for isolating different populations of hair follicles stem cells and epidermal keratinocytes, employing enzymatic digestion of mouse dorsal skin followed by FACS analysis.
FAQs
Exposing the dorsal surface creates a direct optical and mechanical route to defined neural regions. Under a stereomicroscope, investigators can distinguish target tissue from surrounding structures and guide fine instruments more precisely. This selective access is important when an experiment depends on relating a particular brain region to its cellular or molecular properties.
Mechanical damage is a central experimental variable because it can compromise the tissue selected for analysis. Careful handling with fine instruments helps maintain the structural organization needed for imaging, immunostaining, and molecular assays. Reducing unnecessary disruption therefore improves the connection between observed results and the original anatomy of the dorsal brain region.
The physiological buffer provides the transfer environment for the separated tissue after dissection. Moving the sample into an appropriate buffer while minimizing mechanical stress supports its use in downstream experimental analyses. This step is especially relevant when researchers need to examine cellular organization, gene expression, or other properties of a defined neural region.
The isolated preparation emphasizes direct access to a defined dorsal region rather than analysis of the surrounding structures as a whole. Separating the target tissue can make selected anatomy easier to examine with imaging, staining, or molecular methods. As a result, researchers can connect localized structural features with region-specific biological measurements.
A typical workflow begins by exposing the dorsal brain surface under a stereomicroscope. Researchers then remove overlying tissues with fine instruments, separate the selected dorsal tissue from neighboring structures, and transfer it into an appropriate physiological buffer. Throughout the process, controlled manipulation is used to limit mechanical damage before downstream analysis.
The preparation supports several complementary analyses, including imaging, immunostaining, and molecular assays. Imaging can document tissue organization, immunostaining can help examine cellular features, and molecular assays can assess biological properties such as gene expression. Using these approaches together allows anatomy, cellular organization, and molecular patterns to be studied in the same defined context.
Dorsal Brain Isolation is useful in developmental studies because it gives researchers improved access to defined neural regions during analysis. The isolated tissue can be examined for relationships among anatomy, gene expression, and cellular organization. These measurements help experimental biologists investigate how regional structure and molecular characteristics correspond during brain development.