Double Thymidine Block
Double Thymidine Block - Related Videos
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DNA Synthesis Assay: A Technique to Assess DNA Synthesis in Proliferating Cells Using Radioactive Tritiated Thymidine Incorporation
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In this video, we determine the proliferative capacity of neuronal progenitor cells by measuring the incorporation of radioactive tracer tritiated thymidine into the DNA of cells. This technique can be used to study cell proliferation after treatment with certain drugs and growth factors.
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Source: Zeynalov, E. et. al., Continuous IV Infusion is the Choice Treatment Route for Arginine-vasopressin Receptor Blocker Conivaptan in Mice to Study Stroke-evoked Brain Edema. J. Vis. Exp. (2016)This video demonstrates the intravenous delivery of an arginine-vasopressin (AVP) receptor antagonist in a mouse model. Transient middle cerebral artery occlusion induces brain edema. An AVP receptor antagonist is administered through a catheter in the left jugular vein, alleviating edema.
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Immunofluorescent Detection of Two Thymidine Analogues (CldU and IdU) in Primary Tissue
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Cited by 48 •
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We have derived a strategy to detect sequential incorporation of thymidine analogues (CldU and IdU) into tissues of adult mice to quantify two successive rounds of cell division. This strategy is useful to detect cell turnover of long-lived tissues, oncogenic transformation, or transit-amplifying cells.
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Depolarizing Blockers: Pharmocokinetics
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Depolarizing blockers are administered through intravenous injection. Succinylcholine is the most common choice of depolarizing blockers in emergency clinical practices. Although they have a rapid onset, they readily diffuse away from the motor end plate into the extracellular fluid. They are metabolized by enzymes such as liver butyrylcholinesterase and plasma pseudocholinesterases. This produces a short duration of action, typically 5-10 minutes long, unlike nondepolarizing blockers, which...
FAQs
The two exposures create a staged synchronization rather than relying on a single treatment. Cells first encounter excess thymidine, then undergo a thymidine-free growth period before the second exposure. This repeated sequence enriches the population at a common cell-cycle position, making subsequent measurements of replication, regulation, or chromosome behavior easier to align in time.
Excess thymidine disrupts the nucleotide balance needed for DNA synthesis. This imbalance temporarily prevents cells from progressing through S phase, the part of the cycle associated with DNA replication. In the double-treatment design, that temporary restriction helps place more cells at a comparable stage for experiments that depend on timing.
The thymidine-free interval separates the two exposures and provides the period of growth specified by the method. Rather than treating the culture continuously, investigators allow this intervening phase before applying excess thymidine again. That sequence is important when the goal is to obtain a more coordinated cell-cycle population after the second block is removed.
A practical workflow begins with cultured cells, applies excess thymidine, permits thymidine-free growth, and then gives a second excess-thymidine treatment. After the second block is removed, cells re-enter the cycle in a more synchronized manner. Researchers can then analyze events according to the timing of this renewed cell-cycle progression.
Double Thymidine Block is useful when biology experiments require cell-cycle timing to be more coordinated across a cultured population. The synchronized release supports analysis of DNA replication, cell-cycle regulation, gene expression, chromosome behavior, and responses to experimental treatments. It therefore serves as a preparation step for comparing events at different points after progression resumes.
After release, researchers can examine DNA replication, cell-cycle regulation, gene expression, chromosome behavior, or responses to experimental treatments as cells resume coordinated progression. The resulting timing information helps compare events across the cycle and connect molecular or chromosome changes with a defined stage of cell-cycle progression.