Dsrna Treatment
Dsrna Treatment - Related Videos
02:37
Detection of Zika Viral dsRNA Replication Intermediates Using Immunocytochemistry
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2026
Source: Contreras, D., et al. Zika Virus Infectious Cell Culture System and the In Vitro Prophylactic Effect of Interferons. J. Vis. Exp. (2016)The video demonstrates the detection of Zika viral dsRNA intermediates in infected epithelial cells using immunocytochemistry. Infected cells are fixed and then blocked with a blocking solution. The primary and secondary antibodies are added. The nuclei are stained with a fluorescent dye. The labeled viral dsRNA is then seen under a fluorescence...
03:24
RNAi Plating for C. elegans Feeding: A Technique to Induce Target dsRNA Expression in E. coli
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2023
This video describes the principles behind RNAi treatment in C. elegans and demonstrates a protocol to knockdown lin-35 in a transgenic worm strain.
04:24
Injection of dsRNA into Female A. aegypti Mosquitos
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Cited by 8 •
2007
Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.
08:41
Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies
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Cited by 5 •
2018
This protocol describes a microinjection methodology that we have standardized and used for several years to deliver specific quantities of nucleic acids directly to the hemolymph of mosquitoes and house flies. This protocol results in minimal injection mortality and allows dose correlated measurements of fecundity.
08:30
RNAi Interference by dsRNA Injection into Drosophila Embryos
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Cited by 6 •
2011
RNA interference has been proven very effective to analyze gene function in Drosophila tracheal development. A detailed protocol used by Jiang lab to inject dsRNA into fly embryos to knockdown gene expression is illustrated. This technique has the potential for screening genes required for tissue and organ development in Drosophila.
FAQs
Dicer first processes double-stranded RNA into small interfering RNAs. These molecules then guide the RNA-induced silencing complex by sequence complementarity toward matching messenger RNA. Recognition can promote messenger RNA degradation or prevent its translation, linking dsRNA processing to reduced gene expression and allowing researchers to connect a selected RNA sequence with a specific silencing outcome.
Target sequence selection determines which messenger RNA can be recognized by the silencing machinery. Strong matching supports selective reduction of the intended transcript, whereas unintended matching may silence other genes. Designing and evaluating the target sequence is therefore central to determining whether an observed biological change reflects the intended gene or an unintended silencing effect.
Delivery and cellular uptake strongly influence the effectiveness of the treatment, because dsRNA must reach cells before its sequence-specific activity can affect gene expression. The selected target sequence also matters. Consequently, weak or variable silencing may result from limited uptake, ineffective targeting, or unintended gene-silencing effects rather than from the molecular mechanism alone.
An experiment should begin with a target sequence chosen for the gene of interest, followed by delivery of dsRNA under conditions that permit cellular uptake. Researchers can then assess the resulting change in gene expression and examine whether unintended silencing occurred. These checkpoints help distinguish poor delivery from an unsuitable target or off-target activity.
Researchers use this approach to investigate gene function by selectively reducing expression and observing the resulting biological consequences. It can also help model disease-related pathways and evaluate potential therapeutic strategies. Its value comes from connecting a defined sequence-level intervention with changes in cellular or pathway behavior, rather than only measuring gene activity without perturbation.
Unexpected or partial results should be considered in light of delivery, cellular uptake, target sequence selection, and unintended gene-silencing effects. Weak reduction may reflect inadequate entry or an ineffective target, while broader changes may indicate unintended activity. Reviewing these factors prevents researchers from attributing every observed phenotype directly to the intended gene.