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JoVE Journal Biology
Injection of dsRNA into Female A. aegypti Mosquitos
 

Injection of dsRNA into Female A. aegypti Mosquitos

Article doi: 10.3791/215
July 4th, 2007 •
1, 1, 2
1Department of Molecular Biology and Biochemistry, University of California, Irvine (UCI), 2Department of Molecular Biology and Biochemistry, Department of Microbiology and Molecular Genetics, University of California, Irvine (UCI)

Summary
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July 4th, 2007

Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.

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