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Injection of dsRNA into Female A. aegypti Mosquitos
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Injection of dsRNA into Female A. aegypti Mosquitos

Injection of dsRNA into Female A. aegypti Mosquitos

DOI:
10.3791/215-v

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04:24 min

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July 04, 2007

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, ,
1Department of Molecular Biology and Biochemistry,University of California, Irvine (UCI), 2Department of Molecular Biology and Biochemistry, Department of Microbiology and Molecular Genetics,University of California, Irvine (UCI)

Chapters

  • 00:07Introduction
  • 00:37Preparation of Needles for Injection of dsRNA
  • 01:35The Injection Setup
  • 02:47Injection of dsRNA
  • 03:55Conclusion
  • 04:17End Credits

Summary

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Reverse genetic approaches have proven extremely useful for determining which genes underly resistance to vector pathogens in mosquitoes. This video protocol illustrates a method used by the James lab to inject dsRNA into female A. aegypti mosquitoes, which harbor the dengue virus. The technique for calibrating injection needles, manipulating the injection setup, and injecting dsRNA into the thorax is illustrated.

Tags

DsRNA InjectionReverse Genetic ApproachA. Aegypti MosquitoesVector PathogensJames LabDengue VirusCalibrating Injection NeedlesManipulating Injection SetupInjecting DsRNAThorax

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