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Environment

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Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies
 

Reproducible dsRNA Microinjection and Oviposition Bioassay in Mosquitoes and House Flies

Article doi: 10.3791/58650
November 8th, 2018

Summary November 8th, 2018

This protocol describes a microinjection methodology that we have standardized and used for several years to deliver specific quantities of nucleic acids directly to the hemolymph of mosquitoes and house flies. This protocol results in minimal injection mortality and allows dose correlated measurements of fecundity.

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