Ear Vein Sampling
Ear Vein Sampling - Related Videos
07:11
Sampling Blood from the Lateral Tail Vein of the Rat
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Cited by 82 •
2015
Blood samples are useful for assessing biomarkers of physiological states or disease in vivo. Here we describe the methodology to sample blood from the lateral tail vein in the rat. This method provides rapid samples with minimal pain and invasiveness.
09:10
Ear Exam
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2023
Source: Richard Glickman-Simon, MD, Assistant Professor, Department of Public Health and Community Medicine, Tufts University School of Medicine, MA This video describes the examination of the ear, beginning with a review of its surface and interior anatomy (Figure 1). The cartilaginous auricle consists of the helix, antihelix, earlobe, and tragus. The mastoid process is positioned just behind the earlobe. The slightly curving auditory canal ends at the tympanic membrane, which transmits sound...
03:52
Subclavian Vein Blood Sampling in Conscious Rats
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2023
Here we present a combination of effective rat restriction and subclavian vein puncture methods that enable rapid, safe, and repeated blood collection in rats without anesthesia.
06:00
Modified Tail Vein and Penile Vein Puncture for Blood Sampling in the Rat Model
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Cited by 6 •
2023
Here, we present a protocol to offer rapid, easy, and reliable blood collection alternatives for the rat model. We describe three different blood sampling methods according to the context: tail vein puncture under anesthesia or on a conscious animal, and dorsal penile vein puncture under anesthesia.
04:52
Repetitive Blood Sampling from the Subclavian Vein of Conscious Rat
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Cited by 2 •
2022
The present protocol describes a simple and efficient method for collecting blood from the subclavian vein in rats. It enables rapid, timely, and easily identifiable sampling without anesthesia and obtains high-quality blood through repetitive sample collection.
FAQs
The technique relies on the ear’s superficial venous anatomy: the auricular vein can be located near the surface and punctured to obtain a limited blood volume. Because the collection targets a small vessel and is intended to minimize tissue disruption, it can support serial sampling when biological measurements need to be followed over time.
Appropriate handling and aseptic technique are part of obtaining a usable sample while limiting unnecessary disruption to the subject. Handling supports controlled restraint and access to the vessel, whereas aseptic practice helps keep the collection procedure suitable for biological analysis. Together, these conditions improve the practicality of repeated measurements in laboratory animals.
Its main distinction is the combination of superficial venous access, a small sample, and limited tissue disruption. This makes the technique a practical alternative when investigators need blood for analysis but want to reduce reliance on more invasive collection methods. The approach is especially useful when measurements must be repeated during disease or treatment monitoring.
A basic workflow begins with appropriate restraint, followed by locating the auricular vein. The vessel is then punctured with a sterile needle or lancet, and the blood is allowed to enter a collection tube or capillary device. These steps connect subject handling with recovery of a sample suitable for subsequent biological analysis.
The collection setup can use either a sterile needle or lancet for vessel puncture, paired with a collection tube or capillary device to receive the blood. The procedure therefore involves a puncture instrument and a receiving format. These materials support collection of a small sample for downstream hematology, chemistry, or biomarker analysis.
It is useful when investigators need blood-based readouts such as hematology, blood chemistry, or biomarker measurements. The same sampling approach can also support disease monitoring and assessment of treatment-related changes. Its value lies in providing a practical route to obtain biological samples over repeated time points while helping reduce the need for more invasive collection.