Modified Tail Vein and Penile Vein Puncture for Blood Sampling in the Rat Model

Laura Charl&#232;s; Thomas Agius; Irina Filz von Reiterdank; Janna Hagedorn; Yanis Berkane; Hyshem H. Lancia; Basak E. Uygun; Korkut Uygun; Curtis L. Cetrulo Jr.; Mark A. Randolph; Alexandre  G. Lellouch

doi:10.3791/65513

Journal

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Medicine

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Modified Tail Vein and Penile Vein Puncture for Blood Sampling in the Rat Model

JoVE Journal
Medicine

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JoVE Journal Medicine

Modified Tail Vein and Penile Vein Puncture for Blood Sampling in the Rat Model

5,722 Views

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06:00 min

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June 30, 2023

DOI:

10.3791/65513-v

DOI:

10.3791/65513-v

•

06:00 min

June 30, 2023

•

30 Views

Laura Charlès^1,2,3, Thomas Agius*^4,5, Irina Filz von Reiterdank*^1,2,3,6, Janna Hagedorn^1,2,3, Yanis Berkane^1,2,3,7, Hyshem H. Lancia^1,2,3, Basak E. Uygun^2,3,8, Korkut Uygun^2,3,8, Curtis L. Cetrulo Jr.^1,2,3,9, Mark A. Randolph^1,2,3,9, Alexandre G. Lellouch^1,2,3,8

¹Vascularized Composite Allotransplantation Laboratory,Massachusetts General Hospital, ²Harvard Medical School, ³Shriners Children’s Boston, ⁴Center for Engineering in Medicine and Surgery,Massachusetts General Hospital, ⁵Department of Vascular Surgery,Centre Hospitalier Universitaire Vaudois and University of Lausanne, ⁶Department of Plastic, Reconstructive and Hand Surgery,University Medical Center Utrecht, ⁷Department of Plastic, Reconstructive and Aesthetic Surgery,Rennes University Hospital Center (CHU de Rennes), Rennes 1 University, ⁸Center for Engineering in Medicine and Surgery,Massachusetts General Hospital, ⁹Plastic Surgery Research Laboratory,Massachusetts General Hospital

Transcript

The issue of repeated blood collection methods needs to be addressed since the majority of studies on rats require them. It’s important to take into account that a sampling method can be very efficient in a certain context, but become unsuitable for another study. This protocol aims to offer simple and reliable blood sampling alternatives to researchers.

Both methods can be done by a single operator and require no extensive prior training, which allows for standardized procedure. When an animal is under anesthesia, the effect of isoflurane can lead to vessel spasm and make the tail vein puncture unsuitable for blood collection. Our protocol offers an alternative in this situation, the penile vein puncture, which presents a better success rate and yields significantly more amounts of blood in less time.

Summary

Here, we present a protocol to offer rapid, easy, and reliable blood collection alternatives for the rat model. We describe three different blood sampling methods according to the context: tail vein puncture under anesthesia or on a conscious animal, and dorsal penile vein puncture under anesthesia.