Electron Gun
Electron Gun - Related Videos
05:44
Gene-gun Transfection of Hippocampal Neurons
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Cited by 2 •
2006
12:02
Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
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Cited by 8 •
2014
This work describes a novel method for selectively targeting subcellular organelles in plants, assayed using the BioRad Gene Gun.
17:42
Preparation of Gene Gun Bullets and Biolistic Transfection of Neurons in Slice Culture
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Cited by 90 •
2008
We describe a method for preparing DNA coated gold bullets and demonstrate the use of such bullets to biolistically transfect neurons in cultured hippocampal slices.
05:33
Biolistic-Mediated Gene Transfer: A Technique to Deliver Gene of Interest in Target Cells via a Biolistic Gene Gun
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2025
In this video, we demonstrate the protocol of biolistic gene gun-mediated gene delivery in cultured cells. This method provides a simple method of stable genetic transformation using a minimal quantity of DNA.
10:39
Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun
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Cited by 18 •
2010
This article illustrates how to properly use the BioRad Helios Gene Gun to introduce plasmid DNA into onion epidermal cells and how to test for protein-protein interactions in onion cells based on the principle of Bimolecular Fluorescence Complementation...
FAQs
After emission, electric fields accelerate the electrons and focus them into a narrow, directed beam. Acceleration supplies the controlled beam needed for interaction with a specimen, while focusing concentrates it for examination at very small scales. Together, these steps determine how effectively the instrument can interrogate biological structure.
The electron gun’s output must remain controlled because biological imaging depends on a directed beam interacting with the specimen in a consistent way. This control supports the formation of detailed images rather than an unfocused examination. In electron microscopy, beam quality therefore connects the gun’s operation with the visibility of cellular, tissue, and subcellular morphology.
In transmission electron microscopy, image formation uses electrons that pass through the biological specimen. In scanning electron microscopy, the image derives from signals emitted from the specimen after beam interaction. Thus, the same electron source supports different imaging pathways, with transmitted electrons and emitted signals providing distinct routes to examine morphology.
A biological imaging workflow begins with electron generation by thermionic or field emission. Electric fields then accelerate and focus the electrons into a narrow beam, which is directed at the specimen. The instrument uses either transmitted electrons or emitted signals from that interaction to create an image of cellular or subcellular structure.
Electron-gun-based microscopy enables researchers to examine ultrastructure, meaning biological organization at very small scales. The resulting images can reveal cellular organization and specimen morphology, making the approach useful for studying how disease-related changes appear in cells and tissues. Its value lies in linking fine structural detail with biological interpretation.
Applications extend across cells, tissues, and subcellular components. In each case, the focused beam interacts with the specimen and produces information through transmitted electrons or emitted signals. This enables researchers to examine morphology at multiple levels of biological organization and investigate structural alterations associated with disease, rather than limiting analysis to whole-cell appearance.