Embryo Loading
Embryo Loading - Related Videos
02:29
Isolation and Cultivation of Bacteria from Zebrafish Embryos to Assess Infection Load
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2026
Source: Bernut, A., et al., Deciphering and Imaging Pathogenesis and Cording of Mycobacterium abscessus in Zebrafish Embryos. J. Vis. Exp. (2015).This video demonstrates a protocol for isolating and quantifying Mycobacterium abscessus from an infected zebrafish embryo. The procedure involves embryo euthanasia, tissue lysis, and homogenization to release the bacteria, followed by centrifugation and resuspension in a surfactant buffer. Finally, the bacterial suspension is serially diluted, plated...
04:37
Left Atrial Ligation in the Avian Embryo as a Model for Altered Hemodynamic Loading During Early Vascular Development
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Cited by 3 •
2023
Here, we present a detailed visual protocol for executing the left atrial ligation (LAL) model in the avian embryo. The LAL model alters the intracardiac flow, which changes wall shear stress loading, mimicking hypoplastic left heart syndrome. An approach to overcome the challenges of this difficult microsurgery model is presented.
02:31
Rabbit Embryo Thawing: A Warming Procedure to Recover Cryopreserved Rabbit Embryos for Embryo Transfer
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2025
This video demonstrates the procedure for thawing vitrified rabbit embryos and the precautionary measurement steps for optimal embryo recovery.
04:18
Intravenous Microinjection of Nephrotoxins into Zebrafish Embryo: A Technique to Deliver Nephrotoxic Agents into the Bloodstream of Zebrafish Embryo
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2023
This video demonstrates the intravenous microinjection of nephrotoxins into the tail vein of a zebrafish embryo to study their chemical effects on renal function.
05:51
Microinjection of Live Drosophila Embryos: Early Delivery of Reagents to the Developing Embryo
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2023
This video describes how to perform microinjections that deliver reagents into live Drosophila embryos. Researchers can use this method to introduce exogenous compounds like nucleic acids and proteins during the early stages of embryo development. The featured protocol demonstrates how to set up and perform the procedure for injection of any soluble reagent–here, a fluorescent protein for live imaging experiments.
FAQs
During loading, the culture medium surrounds the embryo in the catheter and helps maintain conditions considered suitable for viability. Its limited volume also supports positioning rather than allowing the embryo to move unpredictably within the device. This combination matters because the embryo must remain protected while the catheter is prepared for controlled release into the uterus.
Careful handling limits mechanical stress during the transition from embryo culture to transfer. It also helps prevent the embryo from being lost before it reaches the uterus. These protective effects depend on maintaining the embryo within the catheter and managing the loading step accurately, making technique quality important for preserving the embryo’s condition before release.
Controlled release helps place the embryo accurately after the catheter has been introduced into the uterus. Accurate placement is important because embryo loading connects the laboratory preparation step with the transfer environment, where implantation outcomes are ultimately studied. An uncontrolled release could undermine the intended positioning and make the transfer less consistent.
Embryo Loading forms the operational link between embryo culture in the laboratory and introduction into the uterus during an assisted reproductive procedure. The embryo must leave its culture setting while remaining in suitable conditions and protected from unnecessary mechanical stress. This transition allows laboratory-prepared embryos to proceed into the clinical transfer stage.
At a high level, preparation includes placing the embryo into a transfer catheter with a small volume of culture medium, positioning it appropriately, and then introducing the catheter into the uterus for release. Each stage supports a different goal: maintaining viability, preventing embryo loss, and achieving accurate placement during the transfer.
The critical conditions are the embryo’s surrounding culture medium, its position inside the catheter, the amount of medium used, and the handling needed to avoid mechanical stress. The process must preserve suitable conditions for viability while also allowing controlled release. Managing these factors helps maintain continuity between laboratory preparation and uterine transfer.
This technique is used during assisted reproductive procedures, particularly in in vitro fertilization, when an embryo moves from laboratory culture toward transfer into the uterus. It is relevant whenever the embryo must be handled within a catheter before placement. The method therefore occupies a practical position between laboratory embryo preparation and clinical reproductive care.
Studying the loading step can help researchers consider how embryo handling, mechanical stress, positioning, and release relate to transfer quality and implantation outcomes. Because the technique connects culture with uterine placement, it provides a point for examining factors that may influence this transition. Its significance is therefore both procedural and relevant to reproductive biology research.