Orthotopic Model
Orthotopic Model - Related Videos
08:43
Orthotopic Mouse Model of Colorectal Cancer
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Cited by 109 •
2007
Two techniques can be used to establish this model: injection of a cancer cell suspension into the cecal wall or transplantation of a piece of subcutaneous tumor onto the cecum. This model is useful for studying the natural progression of colorectal cancer and testing new therapeutic agents against colorectal cancer.
07:01
An Orthotopic Mouse Model of Anaplastic Thyroid Carcinoma
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Cited by 10 •
2013
Generation of an orthotopic mouse model of anaplastic thyroid carcinoma is described here. This technique employs surgical placement of human anaplastic thyroid cancer cells into the thyroid of immunodeficient mice, thus creating a more clinically relevant setting to study disease progression as well as screen innovative therapeutic interventions.
09:07
An Orthotopic Model of Murine Bladder Cancer
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Cited by 24 •
2011
Bladder tumors are established in female mice in a minimally invasive fashion through catheterization, local cauterization, and subsequent adhesion of carcinoma cells to the burn sites.
06:48
Fluorescent Orthotopic Mouse Model of Pancreatic Cancer
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Cited by 5 •
2016
A procedure to implant green fluorescent protein-expressing pancreatic cancer cells (PANC-1 GFP) orthotopically into the pancreas of Balb-c Ola Hsd-Fox1nu mice to assess tumor progression and metastasis is presented here.
09:25
Bioluminescent Orthotopic Model of Pancreatic Cancer Progression
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Cited by 34 •
2013
Improved understanding of pancreatic cancer biology is critically needed to enable the development of better therapeutic options to treat pancreatic cancer. To address this need, we demonstrate an orthotopic model of pancreatic cancer that permits non-invasive monitoring of cancer progression using in vivo bioluminescence imaging.
FAQs
The target organ’s surrounding cells, blood vessels, and tissue structures can alter how disease material grows, invades nearby regions, or responds to treatment. These local interactions provide biological context that is absent or different when material is placed elsewhere. As a result, observations may better reflect disease behavior within its naturally affected organ.
The comparison helps determine how strongly anatomical location shapes disease behavior. A non-native site may support growth but may not reproduce the organ-specific interactions that influence progression, invasion, metastasis, or treatment response. An orthotopic model therefore adds context for judging whether findings depend on the disease itself, its surrounding tissue, or both.
By retaining the relevant tissue environment, the system can support investigation of tumor growth, local invasion, metastasis, transplantation, and therapeutic responses as related but distinct outcomes. Researchers can examine disease progression together with interactions among the introduced material and the organ’s surrounding structures, rather than treating growth alone as the primary result.
The experimental material may consist of cells, tissues, or disease material, and it is placed in the anatomical site corresponding to where the condition naturally develops. This arrangement preserves contact with the relevant organ environment. The choice of introduced material and its native location determines which disease or transplantation process the model can investigate.
Researchers may choose this approach when anatomical context is important for studying disease progression, tumor invasion, metastasis, transplantation, or responses to therapy. It is especially relevant when a non-native implantation site could omit interactions with local cells, vessels, or tissue structures. The model can therefore address questions requiring biologically relevant organ-level conditions.
Because treatment responses occur within the tissue environment associated with the condition, an orthotopic model can provide more realistic evidence about therapeutic effects than a non-native setting may provide. These findings can help researchers assess disease behavior and treatment response before clinical investigation, while preserving the biological context needed to interpret those outcomes.