Ex Vivo Muscle
Ex Vivo Muscle - Related Videos
09:34
In Vivo Canine Muscle Function Assay
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Cited by 19 •
2011
We describe a minimally-invasive and painless method to measure canine hindlimb muscle strength and muscle response to repeated eccentric contractions.
14:36
Evaluation of Muscle Function of the Extensor Digitorum Longus Muscle Ex vivo and Tibialis Anterior Muscle In situ in Mice
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Cited by 75 •
2013
Changes in limb muscle contractile and passive mechanical properties are important biomarkers for muscle diseases. This manuscript describes physiological assays to measure these properties in the murine extensor digitorum longus and tibialis anterior muscles.
14:02
Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles
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Cited by 53 •
2012
We describe a method to directly measure muscle force, muscle power, contractile kinetics and fatigability of isolated skeletal muscles in an in vitro system using field stimulation. Valuable information on Ca2+ handling properties and contractile machinery of the muscle can be obtained using different stimulating protocols.
08:33
In Vivo Imaging of Muscle-tendon Morphogenesis in Drosophila Pupae
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Cited by 17 •
2018
Here, we present an easy-to-use and versatile method to perform live imaging of developmental processes in general and muscle-tendon morphogenesis in particular in living Drosophila pupae.
15:56
DNA Transfection of Mammalian Skeletal Muscles using In Vivo Electroporation
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Cited by 99 •
2009
We describe detailed procedures for the efficient transfection of plasmid DNA into the fibers of foot muscles of live mice using electroporation and the subsequent visualization of protein expression using fluorescence microscopy.
FAQs
Electrical stimulation provides a controlled way to activate the isolated tissue and examine its mechanical response. By varying stimulation patterns, investigators can observe changes in contraction, shortening, and fatigue. These measurements connect the imposed activation conditions with muscle performance, helping reveal how tissue-level function responds under defined experimental settings.
The oxygenated physiological solution supports the tissue while it is maintained outside the organism, and its composition can be adjusted as part of the experiment. Temperature, nutrients, and drugs are likewise controllable conditions. This flexibility lets researchers test how defined environmental or pharmacological changes alter muscle responses without changing multiple variables at once.
Compared with studying muscle only within an intact organism, Ex Vivo Muscle offers more direct control over temperature, nutrients, drugs, and stimulation patterns. At the same time, the preparation retains important properties of intact muscle. This combination makes it useful for separating tissue responses to selected conditions while preserving biologically relevant performance.
The basic workflow starts with dissecting the muscle tissue and transferring it into an oxygenated physiological solution. Researchers then apply electrical stimulation and record a selected performance measure, such as force or shortening. Repeated observations can also track fatigue, allowing the experiment to connect preparation conditions, stimulation, and mechanical outcome.
Measurements of force, shortening, and fatigue capture different aspects of muscle performance under the same controlled preparation. Comparing these outcomes can show whether a condition primarily changes the strength of contraction, the extent of shortening, or the ability to sustain performance. Such patterns provide evidence about cellular and tissue-level influences on function.
Applications span muscle physiology, biomechanics, exercise biology, neuromuscular disease, and pharmacology. The preparation is valuable when a study needs direct evidence linking controlled conditions to muscle performance. Researchers can examine responses to drugs or stimulation patterns, or compare functional behavior in investigations of disease-related and exercise-related mechanisms.