Extracellular Interactions
Extracellular Interactions - Related Videos
12:30
Avidity-based Extracellular Interaction Screening (AVEXIS) for the Scalable Detection of Low-affinity Extracellular Receptor-Ligand Interactions
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Cited by 56 •
2012
AVEXIS is a high throughput protein interaction assay developed to systematically screen for novel extracellular receptor-ligand pairs involved in cellular recognition processes. It is specifically designed to detect transient protein interactions that are difficult to identify using other high throughput approaches.
05:51
Coculture Analysis of Extracellular Protein Interactions Affecting Insulin Secretion by Pancreatic Beta Cells
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Cited by 3 •
2013
Transcellular protein interactions are important determinants of pancreatic beta-cell function. Detailed here is a method—adapted from a coculture model of synaptogenesis—for investigating how specific transmembrane proteins influence insulin secretion. Transfected HEK293 cells express proteins of interest; beta cells do not need to be transfected or otherwise directly perturbed.
08:59
Human Dupuytren's Ex Vivo Culture for the Study of Myofibroblasts and Extracellular Matrix Interactions
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Cited by 3 •
2015
Dupuytren’s disease (DD) is a fibroproliferative disease of the palm of the hand. Here, we present a protocol to culture resection specimens from DD in a three-dimensional (3D) culture system. Such short-term culture system allows preservation of the 3D structure and molecular properties of the fibrotic tissue.
03:15
Fluorescence Microscopy of Neutrophil Extracellular Traps or NETs: A Technique to Observe Adhesive Interaction Between Cancer Cells and NETs
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2023
This video demonstrates the fluorescent staining of neutrophil extracellular traps (NETs), the extracellular DNA components expelled by neutrophils. This technique enables the visualization of adhesive interaction between cancer cells and NETs.
03:05
Time-lapse Fluorescence Microscopy of Neutrophil Extracellular Traps or NETs: A Technique to Analyze Post-adhesion Interaction between Cancer Cells and NETs
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2023
In this video, we analyze the post-adhesion behavior of cancer cells trapped in neutrophil extracellular traps (NETs) by co-culturing low-density neutrophils and gastric cancer cells. The time-lapse video analysis helps monitor various cellular events and study the role of NETs in pathological conditions.
FAQs
Selectivity arises when secreted signals, receptors, adhesion proteins, and extracellular matrix components bind compatible partners. These binding events can activate intracellular signaling pathways, converting information from the external environment into cellular responses. As a result, cells may alter their attachment, movement, growth, or differentiation according to the extracellular cues they receive.
Receptors detect and respond to extracellular signals, while adhesion proteins help cells attach to neighboring structures or the surrounding matrix. Matrix components provide an external framework with which cells interact. Together, these components connect extracellular conditions to intracellular signaling, enabling coordinated changes in cell behavior and contributing to organized tissue structure.
Changes outside a cell can modify which signals or binding partners are available and how cells interact with their surroundings. Because these contacts influence intracellular signaling, the same cell may change its attachment, movement, growth, or differentiation under different extracellular conditions. This relationship helps explain how tissue organization and disease progression are affected.
Analysis focuses on the contacts among secreted signals, receptors, adhesion proteins, and extracellular matrix components, together with the cellular responses they produce. Researchers examine how these interactions relate to attachment, movement, growth, differentiation, and tissue organization. This approach connects molecular or physical contacts outside cells with broader biological processes such as immune responses and development.
Studying extracellular contacts can clarify how cells organize into tissues, communicate during immune responses, and change behavior during development. It can also reveal how altered extracellular conditions contribute to disease progression. These questions are relevant when investigating how external molecular and physical cues are translated into changes in cellular behavior.
In wound healing, cancer biology, and regenerative medicine, extracellular contacts provide a way to examine how the surrounding environment influences cells. Their analysis can identify relationships between external cues and changes in attachment, movement, growth, or differentiation. The same principles also support research on biomaterials, where extracellular conditions are important for understanding cellular responses.