Fat Body Removal
Fat Body Removal - Related Videos
06:18
Fat Body Organ Culture System in Aedes Aegypti, a Vector of Zika Virus
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Cited by 15 •
2017
The fat body is the central metabolic organ in insects. We present a live organ culture system that enables the user to study the responses of isolated fat body tissue to various stimuli.
10:28
Body Composition and Metabolic Caging Analysis in High Fat Fed Mice
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Cited by 24 •
2018
This protocol describes the use of a body composition analyzer and metabolic animal monitoring system to characterize body composition and metabolic parameters in mice. An obesity model induced by high-fat feeding is used as an example for the application of these techniques.
06:02
Analyzing Starvation-Induced Autophagy in the Drosophila melanogaster Larval Fat Body
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Cited by 6 •
2022
The present protocol describes the induction of autophagy in the Drosophila melanogaster larval fat body via nutrient depletion and analyzes changes in autophagy using transgenic fly strains.
09:25
Transplantation Into the Mouse Ovarian Fat Pad
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Cited by 7 •
2016
We describe an ovarian fad pad transplantation assay suitable for studies of normal and transformed epithelia of the female reproductive tract. The mouse fat pad allows transplantation of large tissue fragments, is easily accessible for surgery and imaging, and provides the most favorable native environment for tissues of Müllerian origin.
03:53
Ovarian Fat Pad Transplantation Assay: An In Vivo Technique to Introduce Cells into Ovarian Fat Pad in Mouse Models
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2023
In this video, we demonstrate an assay for transplantation of experimental cells and tissue fragments into the mouse ovarian fat pad. This method can be used to monitor cell regeneration and tumor transformation via non-invasive techniques.
FAQs
Removing the fat body allows investigators to separate effects that depend on this tissue from responses generated within another tissue itself. This distinction is useful when examining nutrient allocation or hormone signaling, because changes observed after excision can be compared with outcomes in tissues that remain present.
Preserving adjacent organs is essential because the manipulation itself can change the biological readout. Damage to neighboring tissues may produce responses that are incorrectly attributed to loss of the fat body, while rough handling can compromise later microscopy, molecular analysis, or functional experiments. Dissection quality therefore directly affects interpretation.
Separating the fat body from surrounding structures helps investigators prepare other tissues for focused study. Once the tissue is removed, the remaining material can be examined by microscopy, molecular analysis, or functional experiments with less direct contribution from the excised tissue. This makes the manipulation useful for tissue-specific experimental design.
The workflow begins by immobilizing the insect and opening the body wall while maintaining the specimen in buffered saline. Fine forceps or comparable microtools are then used to separate the fat body from neighboring organs and remove it. Each stage requires controlled handling so the intended tissue is excised without unnecessary disruption.
Fine forceps or other microtools provide the precision needed to separate the fat body from neighboring organs during dissection. The specimen is handled in buffered saline after immobilization and opening of the body wall. These conditions support selective removal while helping preserve tissues intended for microscopy, molecular analysis, or functional experiments.
Researchers may choose this manipulation when they need to test fat body-dependent effects, examine nutrient allocation or hormone signaling, or prepare remaining tissues for downstream analysis. The resulting material can support microscopy, molecular analysis, and functional experiments, making the procedure relevant to studies that connect tissue structure with biological function.