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Analyzing Starvation-Induced Autophagy in the Drosophila melanogaster Larval Fat Body

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JoVE Journal Biology

Analyzing Starvation-Induced Autophagy in the Drosophila melanogaster Larval Fat Body

Analyzing Starvation-Induced Autophagy in the Drosophila melanogaster Larval Fat Body

DOI:

10.3791/64282-v

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06:02 min

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August 04, 2022

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Kexin Shi, Chao Tong

¹Ministry of Education Key Laboratory of Biosystems Homeostasis and Protection and Innovation Center for Cell Signaling Network, Life Sciences Institute,Zhejiang University

Chapters

00:04Introduction
00:40Drosophila Crossing and Egg Laying
01:54Amino Acid Starvation Induces Autophagy
02:51Third Instar Larvae Dissection and Sample Tissue Processing
04:29Results: The Patterns of GFP‐Atg8a Puncta in Control, Mu1, and Mu2 Clones
05:22Conclusion

Summary

Automatic Translation

The present protocol describes the induction of autophagy in the Drosophila melanogaster larval fat body via nutrient depletion and analyzes changes in autophagy using transgenic fly strains.

Analyzing Starvation-Induced Autophagy in the Drosophila melanogaster Larval Fat Body

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Chapters

Summary

Automatic Translation

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