Fibronectin
Fibronectin - Related Videos
01:25
Fibronectins Connect Cells with ECM
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2023
Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal. Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
08:09
Isolation of Chondrocytes and Chondroprogenitors Using Fibronectin Adhesion and Migratory Assay
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Cited by 5 •
2024
This protocol details the isolation of chondrocytes, Fibronectin Adhesion Assay-derived Chondroprogenitors (FAA-CPs), and Migratory Chondroprogenitors (MCPs) from human articular cartilage. It covers enzymatic digestion, fibronectin adhesion, and migration-based assays for isolating and characterizing these cells.
10:57
Examining the Dynamics of Cellular Adhesion and Spreading of Epithelial Cells on Fibronectin During Oxidative Stress
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Cited by 2 •
2019
This method is useful for quantifying the early dynamics of cellular adhesion and spreading of anchorage-dependent cells onto the fibronectin. Furthermore, this assay can be used to investigate the effects of altered redox homeostasis on cell spreading and/or cell adhesion-related intracellular signaling pathways.
07:14
Fabrication of a Biomimetic Nano-Matrix with Janus Base Nanotubes and Fibronectin for Stem Cell Adhesion
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Cited by 20 •
2020
The goal of this protocol is to show the assembly of a biomimetic nanomatrix (NM) with Janus base nanotubes (JBNTs) and fibronectin (FN). When co-cultured with human mesenchymal stem cells (hMSCs), the NMs exhibit excellent bioactivity in encouraging hMSCs adhesion.
09:11
Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification
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Cited by 4 •
2015
Here, we present a protocol to continuously quantify cell adhesion and de-adhesion processes with high temporal resolution in a non-invasive manner by cell-substrate impedance and live cell imaging analyses. These approaches reveal the dynamics of cell adhesion/de-adhesion processes triggered by matrix modification and their temporal relationship to adhesion-dependent signaling events.
FAQs
Fibronectin creates a physical and functional link between cells and matrix components. Its binding to cell-surface integrins connects cellular adhesion machinery with extracellular materials such as collagen. This interaction helps cells remain positioned within tissue while also allowing them to respond to changes in their immediate surroundings.
Mechanical tension can change fibronectin’s molecular arrangement by exposing binding sites that are otherwise less accessible. Once exposed, these sites promote interactions needed for fibril assembly and matrix remodeling. This mechanism connects physical forces in tissues with changes in extracellular matrix organization and helps explain how cells influence their environment.
The organization of fibronectin affects how cells sense and respond to the matrix around them. Because it helps arrange extracellular components and supports cell adhesion and migration, its spatial pattern contributes to tissue structure. Changes in organization can therefore influence matrix remodeling and the maintenance of tissue architecture.
During embryonic development, fibronectin supports the organized cellular interactions required as tissues form. In wound healing, it contributes to cell adhesion, migration, and changes in the surrounding matrix. These roles make fibronectin relevant to both the establishment of tissue structure and the repair of damaged tissue.
Altered fibronectin expression or organization is associated with fibrosis, cancer progression, and impaired repair. These changes may modify how cells interact with their matrix and how the matrix is remodeled. Examining fibronectin therefore provides a way to connect abnormal extracellular organization with changes in tissue behavior and disease progression.
Researchers can use fibronectin to investigate how cells detect and respond to their surrounding matrix. Its interactions with integrins and collagen, together with tension-dependent fibril assembly, provide a framework for examining adhesion, migration, tissue structure, and remodeling. This information is relevant to development, repair, fibrosis, and cancer biology.