Fixed Angle Rotor
Fixed Angle Rotor - Related Videos
09:26
How to Build a Vacuum Spring-transport Package for Spinning Rotor Gauges
0 Views •
Cited by 1 •
2016
Here we describe how to build a robust spring-transport mechanism for a spinning rotor gauge. This device securely immobilizes the rotor and keeps it under vacuum during transportation. We also describe packaging that minimizes the risk of damage during transport. Tests show our design works for typical shocks during transport.
09:45
Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
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Cited by 17 •
2012
Fluorescence Lifetime Imaging (FLIM) has emerged as a key technique to image the environment and interaction of specific proteins and dyes in living cells. FLIM of fluorescent molecular rotors allows mapping of viscosity in living cells.
07:33
Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning
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Cited by 5 •
2010
This technique enables the use of high-resolution magic angle spinning proton MR spectroscopy (HRMAS 1H-MRS) for molecular characterization of live Drosophila melanogaster with a conventional 14.1 tesla spectrometer equipped with an HRMAS probe.
01:13
Contact Angle
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2023
When a solid is dipped inside a liquid, the liquid surface becomes curved near the contact. For some solid–liquid interfaces, the liquid is pulled up along the solid, while for others, the liquid surface is convex or depressed near the solid surface. This phenomenon can be explained using the concept of cohesive and adhesive forces. The adhesive force is the molecular force between molecules of different materials, that is, between the molecules of the solid and the liquid. The cohesive force...
16:31
Fixed Volume or Fixed Pressure: A Murine Model of Hemorrhagic Shock
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Cited by 24 •
2011
The Hemorrhagic Shock model has been a reliable and reproducible resource facilitating the identification and understanding of signaling cascades associated with inflammation and end-organ damage after trauma. This article provides a step-by-step description of surgical and mechanical aspects associated with the Hemorrhagic Shock experimental procedure in mice.
FAQs
The constant tube angle directs sedimenting material toward the outer wall and then the tube bottom. This path concentrates separated particles into a compact pellet rather than leaving them distributed along the tube. The resulting concentration supports clearer recovery of particulate material for subsequent biological or biochemical analysis.
Separation depends on the particles’ size, density, and shape, as well as the centrifugal field applied during the run. These properties influence how readily different materials move outward through the liquid. Consequently, samples containing biologically distinct particles can produce different sedimentation outcomes under the same rotor conditions.
Centrifugal force drives particles outward through the liquid, causing particulate material to sediment away from the rotation axis. The extent of this movement depends on both the applied field and particle characteristics. This mechanism allows suspended biological components to become concentrated separately from the remaining liquid.
A sample is placed in a tube held at the rotor’s constant angle, and centrifugation is applied to generate the centrifugal field. Particles move outward, follow the angled tube toward its outer wall and bottom, and accumulate as a compact pellet. The separated pellet then provides concentrated material for analysis or further preparation.
They are useful when a biological sample must be separated into particulate material for cell fractionation or sample preparation. Depending on particle characteristics and the applied centrifugal field, centrifugation can concentrate cells or organelles into a pellet. That enriched fraction can then support downstream biochemical analysis.
Applications include pelleting cells, organelles, nucleic acids, proteins, and other particulate material. The rotor therefore supports several stages of biological research, from preparing cellular fractions to concentrating molecular components. The material recovered and the separation achieved depend on particle size, density, shape, and the centrifugal field used.