Fluid Phase Uptake
Fluid Phase Uptake - Related Videos
08:02
Reservoir Condition Pore-scale Imaging of Multiple Fluid Phases Using X-ray Microtomography
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Cited by 28 •
2015
We present a methodology for the imaging of multiple fluid phases at reservoir conditions by the use of x-ray microtomography. We show some representative results of capillary trapping in a carbonate rock sample.
07:03
The Ingestion of Fluorescent, Magnetic Nanoparticles for Determining Fluid-uptake Abilities in Insects
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Cited by 3 •
2017
Fluid-feeding insects have the ability to acquire minute quantities of liquids from porous surfaces. This protocol describes a method to directly determine the ability for insects to ingest liquids from porous surfaces using feeding solutions with fluorescent, magnetic nanoparticles.
02:39
Phase Diagrams
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2020
A phase diagram combines plots of pressure versus temperature for the liquid-gas, solid-liquid, and solid-gas phase-transition equilibria of a substance. These diagrams indicate the physical states that exist under specific conditions of pressure and temperature and also provide the pressure dependence of the phase-transition temperatures (melting points, sublimation points, boiling points). Regions or areas labeled solid, liquid, and gas represent single phases, while lines or curves represent...
04:04
Radiolabeled Amino Acid Uptake Assay to Quantify Cellular Uptake of Amino Acids
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2025
This video demonstrates an in vitro technique for estimating amino acid uptake by bone marrow-derived stromal cells. Na+-dependent amino acid transporters mediate the uptake of radiolabeled amino acids inside the cells, and the cellular uptake is estimated by measuring the radioactivity.
03:13
Competence-Induced DNA Uptake in Staphylococcus aureus
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2025
Source: Cafini, F., et.al. Methodology for the Study of Horizontal Gene Transfer in Staphylococcus aureus. J. Vis. Exp. (2017)This video demonstrates competence-induced DNA uptake in Staphylococcus aureus through natural transformation. A chloramphenicol-sensitive strain engineered to constitutively express a sigma factor is cultured under nutrient-limiting conditions to enhance competence, then incubated with donor DNA carrying a resistance gene. Successful gene transfer is confirmed by the...
FAQs
Solutes are generally captured in proportion to their concentration in the surrounding medium rather than selected through a specific binding interaction. As a result, changes in extracellular composition can alter the molecular contents of newly formed vesicles. This concentration-dependent behavior makes the process useful for examining how cells sample and respond to their external environment.
The main distinction is the basis of cargo capture. Fluid phase uptake generally internalizes nearby fluid and dissolved molecules without requiring a particular receptor-cargo match, whereas receptor-mediated endocytosis depends on selective molecular recognition. This difference allows fluid phase uptake to provide information about broad environmental sampling, while receptor-mediated pathways reveal more targeted internalization.
Early endosomes act as an initial sorting compartment for material delivered from the plasma membrane. Internalized contents may be directed toward recycling, degradation, or other stages of membrane trafficking. Following these routes helps connect uptake with membrane turnover and cellular regulation, because the cell can either return components, break them down, or continue processing them.
Measurement provides an indication of how actively cells internalize extracellular fluid and its dissolved contents. Researchers can use this information to compare endocytic activity and examine changes in the movement of material into early endosomes. The resulting assessment supports studies of membrane trafficking and how cells regulate exchanges with their extracellular surroundings.
Studies of fluid phase uptake can address how cells acquire nutrients, maintain membrane turnover, and sense their environment. Tracking the process also helps researchers examine intracellular trafficking after material enters early endosomes. Together, these applications connect vesicle formation at the cell surface with broader questions about cellular interaction with extracellular conditions.
Because dissolved molecules are generally internalized according to their abundance in the surrounding fluid, uptake measurements can reflect both cellular activity and extracellular composition. Interpreting the result therefore requires attention to the medium being sampled. This relationship helps researchers distinguish concentration-dependent capture from selective uptake driven by receptor recognition.