Fluorophore Labeled Probes
Fluorophore Labeled Probes - Related Videos
03:31
Labeling DNA Probes
0 Views •
2021
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions. Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
12:16
Robust 3D DNA FISH Using Directly Labeled Probes
0 Views •
Cited by 49 •
2013
We describe a robust and versatile protocol for analyzing nuclear architecture by 3D DNA FISH using directly labeled fluorescent probes.
07:45
Estimation of Urinary Nanocrystals in Humans using Calcium Fluorophore Labeling and Nanoparticle Tracking Analysis
0 Views •
Cited by 4 •
2021
The objective of this study was to determine whether nanoparticle tracking analysis (NTA) could detect and quantify urinary calcium containing nanocrystals from healthy adults. The findings from the current study suggest NTA could be a potential tool to estimate urinary nanocrystals during kidney stone disease.
11:15
In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
0 Views •
Cited by 16 •
2015
Here a method to localize bacteria within paraffin-embedded tissues using DIG-labeled 16S rRNA-targeting DNA probes has been described. This protocol can be applied to study the role of bacteria in various diseases such as periodontitis, cancers, and inflammatory immune diseases.
08:28
Metabolic Labeling
0 Views •
2023
Metabolic labeling is used to probe the biochemical transformations and modifications that occur in a cell. This is accomplished by using chemical analogs that mimic the structure of natural biomolecules. Cells utilize analogs in their endogenous biochemical processes, producing compounds that are labeled. The label allows for the incorporation of detection and affinity tags, which can then be used to elucidate metabolic pathways using other biochemical analytical techniques, such as SDS-PAGE...
FAQs
The fluorophore absorbs light at a defined excitation wavelength and then emits light at a longer wavelength. This separation allows detection of the probe’s signal against a darker background, so molecules bound to the probe can be distinguished from surrounding cellular or tissue structures during fluorescence-based observation.
Recognition comes from the target-specific component attached to the dye. A nucleic acid probe is suited to selected nucleic acid sequences, whereas an antibody directs detection toward a particular protein. Changing this component changes the molecular target and therefore the biological question the experiment can address.
The same labeling principle can provide different types of information depending on the readout. Fluorescence microscopy shows where a selected molecule occurs within cells or tissues, while flow cytometry supports detection and cell identification. These approaches connect molecular presence with cellular organization and biological context.
A typical workflow begins by pairing a fluorescent dye with a molecule chosen to recognize the biological target. The probe is then assessed in cells or tissues with a fluorescence microscope, flow cytometer, or related imaging method. Detectable signal indicates where the selected target is present or which cells contain it.
They are useful when researchers need to relate a molecular component to its cellular position or distribution. Nucleic acid-based probes can support gene expression analysis, while antibody-based probes can reveal protein localization. The resulting fluorescence pattern helps connect molecular identity with the organization and function of cells or tissues.
Fluorophore-labeled probes can distinguish cells according to selected molecular features and can indicate whether particular molecules are present in a sample. In molecular diagnostics, that targeted signal provides a way to assess molecular composition. In biology, the same information helps interpret differences among cells and their functional states.