Capillary Gel Electrophoresis
Capillary Gel Electrophoresis - Related Videos
08:50
Capillary Electrophoresis (CE)
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2023
Source: Laboratory of Dr. B. Jill Venton - University of Virginia Capillary electrophoresis (CE) is a separation technique that separates molecules in an electric field according to size and charge. CE is performed in a small glass tube called a capillary that is filled with an electrolyte solution. Analytes are separated due to differences in electrophoretic mobility, which varies with charge, solvent viscosity, and size. Traditional electrophoresis in gels is limited in the amount of voltage...
07:31
Two-Dimensional Gel Electrophoresis
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2023
Two-dimensional gel electrophoresis (2DGE) is a technique that can resolve thousands of biomolecules from a mixture. This technique involves two distinct separation methods that have been coupled together: isoelectric focusing (IEF) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). This physically separates compounds across two axes of a gel by their isoelectric points (an electrochemical property) and their molecular weights. The procedure in this video covers the main...
09:22
DNA Gel Electrophoresis
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2023
DNA gel electrophoresis is a technique used for the detection and separation of DNA molecules. An electric field is applied to a gel matrix comprised of agarose, and within the gel, charge particles will migrate and separate based on size. The negatively charged phosphates of the DNA backbone cause DNA fragments to move toward the anode - a positively charged electrode. The video explains the mechanism by which DNA fragments are resolved on an agarose gel, and it provides a step-by-step...
07:54
Analysis of AtHIRD11 Intrinsic Disorder and Binding Towards Metal Ions by Capillary Gel Electrophoresis and Affinity Capillary Electrophoresis
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Cited by 2 •
2018
This protocol combines the characterization of a protein sample by capillary gel electrophoresis and a fast-binding screening for charged ligands by affinity capillary electrophoresis. It is recommended for proteins with a flexible structure, such as intrinsically disordered proteins, to determine any differences in binding for different conformers.
08:25
Using a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
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Cited by 6 •
2017
The genotyping technique described here, which couples fluorescent polymerase chain reaction (PCR) to capillary gel electrophoresis, allows for high-throughput genotyping of nuclease-mediated knockout clones. It circumvents limitations faced by other genotyping techniques and is more cost effective than sequencing methods.
FAQs
Separation depends on the interaction between molecular size, charge, and the matrix. The electric field drives charged molecules through the capillary, while the gel or polymer restricts movement according to size. Molecules therefore migrate at different rates, producing resolved groups that can be interpreted to distinguish components within a biological sample.
Capillary geometry matters because the narrow passage dissipates heat efficiently while an electric field is applied. Better heat management supports stable migration conditions and helps the method achieve rapid separations. This combination is especially useful when biological analyses require efficient processing without relying on large sample quantities.
Charge is essential because the applied electric field moves charged molecules through the capillary. However, movement is also constrained by the matrix, so molecules with different sizes can migrate differently rather than simply traveling together. This combined influence enables the separation of DNA fragments and other charged biomolecules for analysis.
A basic analysis begins by placing a biological sample into the capillary, then applying an electric field to drive its charged components through the gel or polymer matrix. The resulting differences in migration are examined to resolve sample components. Because the method uses little sample and supports automation, this workflow can be incorporated into routine analyses.
It can resolve DNA fragments and support analysis of genetic variation by separating nucleic acid components according to their migration through the matrix. The resulting separation provides a basis for assessing nucleic acid samples and distinguishing molecular components. These capabilities make the technique relevant to biological research and to analyses where fragment-level resolution is important.
Beyond DNA work, the technique can separate proteins and other biomolecules, extending its usefulness across biological sample types. Its automated operation, low sample requirements, and quantitative output support research, clinical analysis, and quality control. In these settings, the result is not only a separation, but also measurable information for evaluating the analyzed sample.