Fraction Collection
Fraction Collection - Related Videos
10:09
Fractional Distillation
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2023
Source: Laboratory of Dr. Nicholas Leadbeater — University of Connecticut Distillation is perhaps the most common laboratory technique employed by chemists for the purification of organic liquids. Compounds in a mixture with different boiling points separate into individual components when the mixture is carefully distilled. The two main types of distillation are "simple distillation" and "fractional distillation", and both are widely used in organic chemistry laboratories. Simple distillation...
07:57
Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery
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Cited by 17 •
2009
The accompanying video describes the use of the GELFREE 8100 Fractionation System, which partitions complex protein samples on the basis of molecular weight and recovers the fractions in liquid phase. The video describes how the technology works, how it is used, and provides resultant data, with polyacrylamide gel electrophoresis analysis of fractionated bovine liver homogenate.
09:01
An Ultra-clean Multilayer Apparatus for Collecting Size Fractionated Marine Plankton and Suspended Particles
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Cited by 6 •
2018
Plankton and suspended particles play a major role in the biogeochemical cycles in the ocean. Here, we provide an ultra-clean, low stress method for the collection of various sizes of particles and plankton at sea with the capability of handling large volumes of seawater.
04:25
Preparation of Subcellular Fractions from Leukemic Cells: A Method to Generate Cellular and Nuclear Fractions from Leukemic Cells
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2023
This video describes the preparation of subcellular fractions from leukemic cells which is a method to generate cellular and nuclear fractions from leukemic cells. The nuclear and cytoplasmic fractions can be used for further downstream analysis.
07:45
Preparation and Fractionation of Xenopus laevis Egg Extracts
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Cited by 7 •
2008
Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.
FAQs
Synchronization links the detector’s observation of eluting material with the collector’s routing decision. A detector monitors material as it leaves the separation system, while an automated valve or collector directs portions into separate tubes or microplates according to elapsed time, eluted volume, or a signal threshold. This coordination preserves the relationship between separation behavior and each collected portion.
Changes in absorbance provide an indication that a compound is emerging from the separation process. These signal changes help identify which portions may contain a target and can guide collection boundaries or later selection. Because absorbance indicates the presence of eluting material rather than confirming its identity, individual portions can be analyzed afterward for biological characterization.
Time-defined fractions are separated according to when material exits the system, whereas volume-defined fractions are separated after specified amounts have eluted. Signal-defined collection uses detector behavior, such as an absorbance change, to identify relevant portions. The choice determines how closely collection follows the separation profile and how precisely researchers can isolate material associated with a target.
Researchers first allow the separated sample to elute while monitoring the emerging material. The collection system then routes successive portions into labeled tubes or microplates using time, volume, or detector-signal criteria. After collection, they inspect individual fractions or select compatible portions for pooling. This workflow connects the separation step with purification assessment or downstream biological analysis.
The approach is useful when separated biological components must be retained for additional analysis or use. Supported applications include protein purification, nucleic acid separation, and metabolite analysis. Collected portions can also move into downstream assays, allowing researchers to examine biological material after separation rather than relying only on the separation profile itself.
Individual-fraction analysis reveals which portions contain material of interest after separation. Researchers can use that information to evaluate the distribution of a target across the collection sequence, identify portions suitable for further work, and determine which samples to pool. The resulting fractions therefore support both biological characterization and decisions about improving sample purity or concentration.