Gel Extraction
Gel Extraction - Related Videos
03:22
SDS-PAGE Based Extraction of Extracellular Vesicles Associated Proteins: A Procedure to Extract Proteins from EVs and Prepare Them for In-Gel Digestion
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2023
In this video, we demonstrate the SDS-PAGE in-gel digestion method for protein extraction from extracellular vesicles or EVs. Once isolated, the obtained protein fragments can be used for proteomic analysis.
08:31
Total Protein Extraction and 2-D Gel Electrophoresis Methods for Burkholderia Species
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Cited by 2 •
2013
Members of the Burkholderia genus are pathogens of clinical importance. We describe a method for total bacterial protein extraction, using mechanical disruption, and 2-D gel electrophoresis for subsequent proteomic analysis.
06:30
Gel Purification
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2023
Gel purification is used to recover DNA fragments after electrophoretic separation. DNA recovery from an agarose gel includes three basic steps: binding, washing and eluting from a silica column. DNA is believed to bind to silica in the presence of high salt via a salt bridge. Following binding, DNA is washed of impurities and eluted under low salt conditions disrupting this interaction. This video goes through a step-by-step, generalized procedure for cutting out a band from the gel, gel...
03:16
Phospholipase Activity Assay: A Gel Diffusion Assay to Determine Phospholipase Activity in Crude Venom Extract from Anthopleura dowii
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2025
In this video, we demonstrate an assay to establish the presence of phospholipase in the crude venom extract from Anthopleura dowii—a marine sea anemone. The assay utilizes an agarose gel diffusion technique, in which the enzyme phospholipase diffuses and breaks down phospholipids incorporated in the solid media in a radial manner.
07:31
Two-Dimensional Gel Electrophoresis
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2023
Two-dimensional gel electrophoresis (2DGE) is a technique that can resolve thousands of biomolecules from a mixture. This technique involves two distinct separation methods that have been coupled together: isoelectric focusing (IEF) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). This physically separates compounds across two axes of a gel by their isoelectric points (an electrochemical property) and their molecular weights. The procedure in this video covers the main...
FAQs
The recovery chemistry uses two sequential changes in the DNA sample. First, dissolving the agarose releases the separated fragment from the gel matrix. The DNA then binds to either a silica membrane or a magnetic matrix, allowing washing to remove gel components and other contaminants. Elution reverses this capture step and produces purified DNA for later manipulation.
Size-based separation allows researchers to distinguish a desired DNA fragment from other products present in the sample. After electrophoresis, the selected band represents the fragment at the chosen position in the agarose gel. Recovering only that band helps remove unwanted products and supports more accurate downstream cloning, sequencing, PCR, or probe-labeling work.
After electrophoresis, researchers use visible or ultraviolet light to locate the band corresponding to the desired DNA size. They excise that region of agarose so the selected fragment enters the purification workflow rather than the entire lane. This selection connects the physical position of a band with the specific DNA population required for a downstream experiment.
The excised agarose is first dissolved to release the DNA fragment. The dissolved sample is then applied to a silica membrane or magnetic matrix, where the DNA is captured. Washing removes remaining gel components and contaminants, and elution releases the purified fragment. This sequence converts a band embedded in gel into DNA suitable for subsequent molecular biology procedures.
Researchers may choose gel extraction when a specific DNA fragment must be isolated from other electrophoretically separated products. The recovered material can support cloning, sequencing, PCR, probe labeling, and other downstream applications. Its value is greatest when the experiment requires a selected fragment rather than an unseparated mixture containing multiple DNA products.
Gel extraction provides a purified DNA fragment selected according to its position and size in an agarose gel. Removing gel components and unwanted products prepares that fragment for accurate analysis or further manipulation. In biology research, this makes the technique useful for connecting electrophoretic separation with experiments that require a defined nucleic acid input.