Gene Identification
Gene Identification - Related Videos
08:17
VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
0 Views •
Cited by 22 •
2013
Virus-induced gene silencing is an useful tool for identifying genes involved in nonhost resistance of plants. We demonstrate the use of bacterial pathogens expressing GFPuv in identifying gene silenced plants susceptible to nonhost pathogens. This approach is easy, fast and facilitates large scale screening and similar protocol can be applied to studying various other plant-microbe interactions.
06:34
Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
0 Views •
Cited by 29 •
2016
Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) and molecular techniques (16S rRNA gene sequencing) permit the identification of rare bacterial pathogens in routine diagnostics. The goal of this protocol lies in the combination of both techniques which leads to more accurate and reliable data.
08:57
Identification of Novel Genes Associated with Alginate Production in Pseudomonas aeruginosa Using Mini-himar1 Mariner Transposon-mediated Mutagenesis
0 Views •
Cited by 13 •
2014
Here we describe a protocol using the mini-himar1 mariner transposon-mediated mutagenesis for generating a high-density insertion mutant library to screen, isolate and identify novel alginate regulators in the prototypic Pseudomonas aeruginosa strain PAO1.
14:12
Rodent Identification II
0 Views •
2015
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN Animal records must be accurately maintained to ensure that data collection is correct. Records range from maintaining information on cage cards to having a detailed database with all of the relevant information on each animal. The primary component of recordkeeping is the individual identification of research animals. There are a variety of methods suitable for identifying mice and rats. This...
05:22
Identification of the Genes Involved in Stomatal Development via Epidermal Phenotype Scoring
0 Views •
2023
This paper describes two phenotyping methods without the use of epidermal peels to characterize the genes controlling stomatal development. The first method demonstrates how to analyze a stomatal phenotype using a toluidine blue O-stained plant epidermis. The second method describes how to identify stomatal ligands and monitor their biological activities.
FAQs
They look for consistent relationships among DNA sequence differences, genetic markers, inheritance patterns, and the observed phenotype. A candidate becomes more convincing when multiple lines of evidence place it in the relevant genomic region and connect it with altered gene expression or biological function. Functional validation then helps test whether the change contributes to the trait or disease rather than merely occurring alongside it.
Genome-wide association studies compare genetic variants across populations to find variants statistically associated with a trait or disease. Linkage analysis follows how markers and traits are inherited within families, helping narrow the responsible genomic region. Because these approaches examine different evidence, researchers can use them alongside DNA sequencing and expression profiles to refine candidate genes and variants.
Expression profiles show where and when genes are active, providing biological context for a suspected association. If expression patterns correspond to the phenotype or disease-related process, they can strengthen the case that a candidate gene has a relevant role. This evidence complements sequence comparisons and inheritance data, helping connect a genomic finding with cellular function.
A typical workflow begins by characterizing the phenotype and examining inheritance patterns or genetic associations. Researchers then narrow a genomic region using markers, compare DNA sequences, and evaluate gene-expression profiles. Candidate genes or variants are prioritized and subjected to functional validation, which helps determine whether they plausibly explain the observed biological trait, function, or disease.
DNA sequencing is useful after an associated genomic region or candidate gene has been identified, because it reveals sequence differences that may explain the phenotype. Researchers can compare those sequences with relevant genetic information and evaluate whether particular variants track with the trait. Sequencing therefore helps move from a broad location to specific candidate changes for further validation.
In biology, identified genes support studies of cellular processes and evolution by linking genetic variation with traits and functions. In medicine, the findings can contribute to disease diagnosis, targeted drug development, and personalized medicine. Gene identification also provides a foundation for genetic engineering, because researchers need relevant genetic information before investigating or modifying biological systems.