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Chapters
- 00:04Introduction
- 00:46Sampling and TBO Staining of Arabidopsis Cotyledons
- 01:58Peptide Treatment and Imaging
- 03:24Results: The Effect of the Incubation Time with TBO on the Genotypes, TBO-Stained Epidermis Images, and Confocal Images of Cotyledon Epidermis with or without Peptide Treatment
- 04:27Conclusion
This paper describes two phenotyping methods without the use of epidermal peels to characterize the genes controlling stomatal development. The first method demonstrates how to analyze a stomatal phenotype using a toluidine blue O-stained plant epidermis. The second method describes how to identify stomatal ligands and monitor their biological activities.
