Genome Remodeling
Genome Remodeling - Related Videos
02:54
Nucleosome Remodeling
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2020
Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure. Nucleosome remodeling complex Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
08:31
Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
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Cited by 2 •
2013
Single Virus Genomics (SVG) is a method to isolate and amplify the genomes of single virons. Viral suspensions of a mixed assemblage are sorted using flow cytometry onto a microscope slide with discrete wells containing agarose, thereby capturing the virion and reducing genome shearing during downstream processing. Whole genome amplification is achieved using multiple displacement amplification (MDA) resulting in genomic material that is suitable for sequencing.
02:02
Genomics
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2020
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
08:31
Genome Editing
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2023
A well-established technique for modifying specific sequences in the genome is gene targeting by homologous recombination, but this method can be laborious and only works in certain organisms. Recent advances have led to the development of “genome editing”, which works by inducing double-strand breaks in DNA using engineered nuclease enzymes guided to target genomic sites by either proteins or RNAs that recognize specific sequences. When a cell attempts to repair this damage, mutations can be...
07:31
A Model of Cardiac Remodeling Through Constriction of the Abdominal Aorta in Rats
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Cited by 26 •
2016
A rat model of abdominal aortic constriction that induces cardiac hypertrophy and remodeling is described. An efficient, highly-reproducible, and minimally-invasive method is used to provide a simple yet useful platform for research in myocardial hypertrophy and dysfunction.
FAQs
Chromatin remodeling changes how DNA is packaged and exposed within the genome. Nucleosome repositioning can make particular genomic regions more or less accessible, while related chromatin changes influence whether regulatory information can be reached. This altered exposure helps determine which genes are available for expression without necessarily changing the underlying DNA sequence.
DNA methylation and histone modification help regulate the functional state of genomic regions. By changing molecular features associated with DNA or its packaging proteins, these processes can influence how accessible particular regions become and how gene expression is controlled. Their effects are especially relevant when cells change identity during development or differentiation.
Some remodeling processes primarily change genome organization and exposure, as seen with chromatin remodeling, methylation, histone modification, and nucleosome repositioning. DNA repair and recombination instead act on the continuity or arrangement of genomic material. This distinction matters because altered access can regulate gene activity, whereas repair or recombination can preserve or reorganize genome structure.
Regulation ensures that genomic regions become accessible or maintained in ways appropriate to changing cellular conditions. During development and cell differentiation, coordinated remodeling supports different cellular programs, while stress responses require corresponding changes in genome regulation. If these controls become disrupted, gene-expression networks may be affected and normal biological responses can fail.
A complete investigation considers both genome organization and genome maintenance. Researchers may examine chromatin state, DNA methylation, histone modification, nucleosome positioning, DNA repair, and recombination, then relate these features to gene expression or cellular behavior. Considering these processes together helps distinguish changes in genomic access from changes affecting genome continuity.
Genome remodeling provides a framework for studying how regulatory states are established and maintained as cells develop. Changes involving DNA methylation, histone modification, and chromatin organization can be examined in relation to stable patterns of gene regulation and changing cell identities. This makes remodeling central to research on epigenetic inheritance and developmental biology.
Abnormal remodeling can disrupt regulatory networks, making it relevant to cancer biology and disease research. Studying these alterations helps researchers connect changes in genome organization, sequence maintenance, or gene regulation with pathological states. The same knowledge supports investigation of genome editing and therapeutic strategies intended to influence or correct biologically important genomic processes.