Gut Epithelium Adhesion
Gut Epithelium Adhesion - Related Videos
02:44
In Vitro Gut Model to Assess Bacterial Adhesion and Internalization
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2026
Source: Calatayud Arroyo, M., et al. Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface. J. Vis. Exp. (2018).This video demonstrates an in vitro gut host–microbe model to study bacterial viability. A co-culture of Caco-2 and HT29-MTX cells is incubated with bacteria pre-treated under simulated digestive conditions. Epithelial barrier integrity is assessed using transepithelial electrical resistance, and fractions from the medium, solubilized...
01:14
Adhesion
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2019
Adhesion occurs when one type of molecule is attracted to a different molecule. Water exhibits adhesive properties in the presence of polar surfaces, such as glass or cellulose in plants. For instance, when water is poured into a glass, the positively charged hydrogen molecules of water are more attracted to the negatively charged oxygen molecules in the silica than to the oxygen in neighboring water molecules. Capillary action is a result of water’s adhesive tendencies. When a narrow glass...
15:27
Extracting DNA from the Gut Microbes of the Termite (Zootermopsis Angusticollis) and Visualizing Gut Microbes
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Cited by 18 •
2007
This video illustrates the technique for extracting DNA from the species of microbes resident in the termite hindgut. The preparation of a wet mount slide, which is useful for visualizing the gut microbial community is also illustrated, and a tour through the species-rich gut environment is given.
09:54
Methods for Rapid Transfer and Localization of Lyme Disease Pathogens Within the Tick Gut
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Cited by 28 •
2011
Lyme disease research studies often require generation of ticks infected with the pathogen Borrelia burgdorferi, a process that typically takes several weeks. Here we demonstrate a microinjection-based tick infection procedure that can be accomplished within hours. We also demonstrate an immunofluorescence method for in situ localization of B. burgdorferi within ticks.
04:09
An Ex Vivo Gut Organ Culture System to Study Host-Microbiota Interactions
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2025
In this video, we describe the methodology for setting up an ex vivo gut organ culture system to study the interactions between mouse intestinal tissue fragments and specific gut bacteria. The device consists of two pairs of input-output ports, the first connecting the intestinal tissue lumen and supplying the specific bacterial suspension into it, and the second continuously supplying media to the device's wells to maintain ex vivo tissue viability.
FAQs
Adherens junctions and desmosomes both connect neighboring intestinal cells, but they contribute to tissue strength through distinct adhesive systems. Adherens junctions help organize cell-cell contact and epithelial structure, whereas desmosomes provide strong mechanical attachment between cells. Their combined activity helps the epithelium withstand continual mechanical stress while preserving a continuous tissue surface.
Tight junctions regulate paracellular permeability, meaning the movement of substances between adjacent epithelial cells rather than through them. This control helps the intestine manage access across the epithelial layer while maintaining cell polarity, the organized distinction between different regions of each cell. Their function therefore links adhesion with selective barrier behavior and epithelial organization.
Integrin-based contacts connect epithelial cells to the basement membrane beneath them, providing anchorage to the underlying extracellular matrix. This cell-matrix relationship supports epithelial organization as cells are renewed and helps the tissue respond when damage requires repair. Studying these contacts clarifies how attachment to the matrix complements cell-cell adhesion during maintenance of the intestinal lining.
The intestinal epithelium faces continual mechanical and chemical stress, so adhesion must preserve both tissue continuity and controlled permeability. Cell-cell junctions provide coordinated attachment, while cell-matrix contacts anchor the layer to its support. Together, these interactions allow the barrier to remain organized as epithelial cells renew and as the tissue responds to local damage.
Research on gut epithelium adhesion can show how epithelial cells maintain attachment while a damaged region is repaired. Examining adherens junctions, desmosomes, tight junctions, and integrin-based contacts provides a framework for relating mechanical cohesion, permeability, polarity, and matrix anchorage to tissue recovery. This makes adhesion relevant to understanding how intestinal barriers are restored after injury.
Altered junctional signaling can contribute to intestinal dysfunction, making adhesion a useful focus in studies of inflammatory disease and infection. Researchers can examine how changes in cell-cell or cell-matrix interactions relate to barrier integrity, epithelial polarity, and the movement of nutrients or microbes. These connections help place junctional behavior within broader biological studies of intestinal health.