Ha-derived Probes
Ha-derived Probes - Related Videos
03:03
Measuring the Activation of Fc-Mediated Effector Functions by HA Antibodies
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2025
This video demonstrates an assay measuring the antibody-mediated activation of T cell effector functions. Adding an influenza virus hemagglutinin (HA)-specific monoclonal antibody to transfected mammalian cells expressing HA results in the formation of immune complexes with HA. Upon introducing engineered T cells expressing Fc receptors and a luciferase reporter, the T cells are activated by the antibody-HA complexes, and this activation is detected by adding a luciferase substrate and...
05:38
Custom-made Microdialysis Probe Design
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Cited by 9 •
2015
Microdialysis is a commonly used technique in neuroscience research. Therefore commercial probes are in great demand.In this work a probe assembly is explained in detail to build a reliable, concentric, custom-made microdialysis probe for less than $10.
13:58
Probing C84-embedded Si Substrate Using Scanning Probe Microscopy and Molecular Dynamics
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Cited by 1 •
2016
This paper reports the nanomaterial fabrication of a fullerene Si substrate inspected and verified by nanomeasurements and molecular dynamic simulation.
07:28
Measurement of Bioelectric Current with a Vibrating Probe
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Cited by 15 •
2011
The manufacture, calibration and use of non-invasive vibrating probes to measure bioelectric current in various biological systems is described.
05:57
Peroxisome Staining in Mammalian Cells Using Peroxisome-Specific Probes
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2025
PeroxiSPY probes are used to detect peroxisomes in mammalian cells. Here we demonstrate how to use the probes to stain peroxisomes in live and fixed cells.
FAQs
Binding to CD44 can concentrate a probe at the cell surface and may trigger receptor-mediated uptake. This creates a link between extracellular recognition and intracellular delivery of an attached payload. As a result, probe localization is not determined only by the label or functional cargo; the hyaluronic acid component contributes to where the tool acts and what cells can be interrogated.
The attached payload determines the probe’s measurable output. Fluorescent or imaging payloads can generate signals for visualizing cells and tissues, whereas functional payloads can produce a biological effect. Hyaluronic acid supplies the CD44-associated targeting interaction, while the cargo supplies the readout or intervention, allowing one recognition strategy to support different experimental purposes.
Probe accumulation can reflect more than passive presence in a sample because hyaluronic acid binding to CD44 may promote selective localization and receptor-mediated uptake. This makes CD44 relevant to interpreting cell-surface activity and internalization. In biological studies, the interaction helps connect a measured signal with receptor-associated behavior rather than treating the probe solely as an inert marker.
An experiment begins by pairing hyaluronic acid with a payload suited to the intended question, such as an imaging label for visualization or a functional cargo for intervention. The resulting construct can then be examined in cells or tissues for localization, uptake, signal generation, or biological effect. This configuration links molecular recognition to the chosen assay outcome.
These probes can support studies of cell-surface receptor activity, tumor-associated biology, inflammation, and drug delivery. Their value comes from combining hyaluronic acid-based recognition with a measurable signal or functional cargo. Consequently, investigators can use them to examine how cells interact with CD44-associated probes, visualize relevant biology, or explore targeted delivery in cellular and tissue contexts.
They can reveal where probe-associated signals occur and help investigators examine cell-surface receptor activity, cellular uptake, or responses to a functional payload. In tissue and cell studies, fluorescent or imaging outputs support visualization and analysis, while functional outputs support targeted intervention. Interpretation therefore depends on both localization through CD44-related recognition and the behavior of the attached payload.