Head Separation Method

Head separation method is a biological dissection technique that physically separates an organism’s head from its trunk or body, allowing researchers to examine region-specific anatomy, physiology, and responses. It works by making a controlled cut at a defined anatomical boundary, then analyzing the separated head and remaining tissues independently or comparing their subsequent changes. This approach supports studies of neural organization, regeneration, development, wound responses, and interactions between head-derived signals and tissues elsewhere in the body.

Head Separation Method - Related Videos

Research

JoVE EoE - Rodent Models

Mouse Headpost Implantation: A Method for Head Fixation in Rodents

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2023

This video shows a headpost implantation surgery in murine model. The headpost is used not only to head-fix and therefore restrain the mouse during experimentation, but also to securely attach a head device in freely moving animals.

A Method to Inflict Closed Head Traumatic Brain Injury in Drosophila

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Cited by 37 •

2015

Here we describe a method to inflict closed head traumatic brain injury (TBI) in Drosophila. This method provides a gateway to investigate the cellular and molecular mechanisms that underlie TBI pathologies using the vast array of experimental tools and techniques available for flies.

A Method to Inflict Closed Head Traumatic Brain Injury in Drosophila

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2025

The video demonstrates the use of a High-Impact Trauma (HIT) device to induce closed-head traumatic brain injury (TBI) in Drosophila. TBI causes mechanical brain trauma affecting neuronal function, which over time leads to neurodegeneration and mortality.

Thin Layer Chromatography-Based Separation of Mycolic Acid Variants: A Method to Separate Mycobacterial Cell Wall Lipids Based on Polarity

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2025

This video demonstrates the thin-layer chromatography-based separation of mycolic acids. This technique separates the different mycolic acids—long-chain lipids present in the cell wall of mycobacteria—based on their polarity.

Education

JoVE Science Education - Chemistry

Separation of Mixtures via Precipitation

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2023

Source: Laboratory of Dr. Ana J. García-Sáez — University of Tübingen Most samples of interest are mixtures of many different components. Sample preparation, a key step in the analytical process, removes interferences that may affect the analysis. As such, developing separation techniques is an important endeavor not just in academia, but also in industry. One way to separate mixtures is to use their solubility properties. In this short paper, we will deal with aqueous solutions. The...

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